益肾活血方对早期糖尿病肾病大鼠作用机制研究

Mechanism Research of Yishenhuoxue Recipe Jether for Early Diabetic Nephropathy in Rats

目的:探讨以益气养阴活血法组方的"益肾活血方"对糖尿病肾病(diabetic nephropathy,DN)模型大鼠的作用机制。方法:用中药煎液灌服DN模型大鼠,生化检测实验大鼠24 h尿蛋白、血肌酐(Scr)、尿素氮(BUN)、血浆白蛋白(ALB)、血脂、血糖;Leica Qwin Plus图像分析系统测量肾小球直径;PAS染色观察大鼠肾脏病理;免疫组织化学检测肾组织TGF-β1表达。结果:该方能够降低实验大鼠24 h尿蛋白、Scr、BUN、升高ALB,减少肾小球直径及肾重,改善肾脏病理、降低肾组织TGF-β1表达,治疗组无论高、低剂量组与模型组相比,均具有统计学意义(P<0.05或P<0.01),但以高剂量组明显(P<0.01)。结论:益肾活血方可能通过影响DN大鼠肾组织中TGF-β1的表达,而改善上述生化指标,减轻肾脏病理损害,且与剂量相关。

Objective：To study the the mechanism of action of yiqi huoxue method in formula ＂＂ benefit of yishenhuoxue recipe＂ diabetic nephropathy （DN） model of of rats. Methods：Using traditional Chinese medicine Fried liquid filling DN model rats, serve biochemical tests 24 h urine protein experimental rats, creatinine （Scr）, urea nitrogen （BUN）, plasma albumin （ALB）, blood fat and blood sugar; Leica Qwin Plus image analysis system;glomerular diameter measuring A PAS stain observation rat kidney pathological;Immunohistochemical （IHC） detection kidney tissues TGF-beta 1 expression. Results：The party can reduce experimental rats 24 h urine protein, Scr, BUN, higher ALB, reduce renal glomerulus diameter and heavy, improve renal pathology, reduce the kidney tissues TGF-beta 1 express,the treatment group whether high and low dose group, compared with model groups was statistically significant（ P 〈 0.05 or P 〈 0. 01 ） ,but in the highdosage groups obvious（ P 〈 O. 01 ）. Conclusion： The benefit of yishenhuoxue recipe may through the effect of rats in the kidney tissues DN TGF-beta 1 expression, and improve the biochemical indicators, reduce kidney pathological damage dose-related.