应用PCR技术诊断新生儿隐匿型先天性弓形虫病

DIAGNOSISONLATENTCONGENITALTOXOPLASMOSISOFNEWBORNSUSINGPCRTECHNIQUE

目的：早期、快速诊断新生儿隐匿型先天性弓形虫病，以便早期实施干预治疗，减少或减轻先天性弓形虫病的后发症状。方法：采用聚合酶链反应（ＰＣＲ）技术检测８６例孕中、晚期血弓形虫ＤＮＡ（ＴＯＸ－ＤＮＡ）阳性孕妇所生新生儿脐血、静脉血、尿液和母乳中ＴＯＸ－ＤＮＡ。结果：脐血、静脉血和尿液ＴＯＸ－ＤＮＡ阳性率分别为５３．４９％、４８．８６％和４１．８６％，而母乳中无１例阳性。结论：应用ＰＣＲ技术早期诊断新生儿隐匿型先天性弓形虫病简便、快速，灵敏度高，特异性强。

Objective: Todiagnosethelatentcongenitaltoxoplasmosis(Tox)ofnewbornsearlyandrapidly,soastocarryoutanearlytherapy,andreduceoreasethelatesymptomsofthecongenitaltoxoplasmosis. Methods: Usingpolymerasechainreaction(PCR),ToxDNAwasassayedincordblood,veinblood,urineandmothersmilkof86newbornswhosemothershadpositiveToxDNAinthemiddleandlateperiodsofpregnancy. Results: Among86newbornsthepositiverateofTOXDNAincordblood,veinbloodandurinewas5349%,4886%and4186%respectively,butnopositivecasewasfoundinmothersmilk. Conclusion: ThePCRtechniqueindetectingTOXDNAwassimple,rapid,sensitiveandspecific.