鹅副粘病毒实时荧光定量RT-PCR检测方法的建立

Real-time quantitative RT-PCR assay for detection of Goose paramyxo virus

根据GenBank所载鹅副粘病毒(GPMV)的F基因保守区序列设计一对特异性引物,应用RT-PCR方法扩增F基因片段,克隆、测序后,采取Taqman探针法建立了检测鹅副粘病毒的荧光RT-PCR方法。结果表明,该方法特异性强,敏感性高,可以用于鹅副粘病毒的检测。

One pair of primers specific based on the conservative reigon of GPMV F designed gene sequences in GenBank was designed F gene was amplified by RT-PCR, and then cloned and sequenced The real-time RT-PCR method was established by using the technology of Taqman probe in order to detect Goose paramyxovirus. The result showed that the method described here with highly specificity and sencificity, which could be used for detection of GPMV.