寻常性银屑病皮损中bcl-2 Fas及FasL的表达

Expression of Some Apoptosis Regulatory Molecules in Psoriasis Vulgaris.

目的 探讨银屑病表皮角质形成细胞异常增殖和分化机制。方法 采用免疫组化技术对１０ 例寻常性银屑病皮损中ｂｃｌ２ 、 Ｆａｓ 及 Ｆａｓ Ｌ 表达进行了检测。结果 在寻常性银屑病皮损中，７ 例表达ｂｃｌ２ ，且表达部位主要位于棘细胞层上方和角质层内角化不全细胞；１０ 例表达 Ｆａｓ ，表达部位与ｂａｌ２相似；浸润炎性细胞均不表达 Ｆａｓ Ｌ。相关分析表明，角质形成细胞ｂｃｌ２ 和 Ｆａｓ 表达强度呈显著负相关（ ｒ ＝ － ０ ．７０７ ， Ｐ＜ ０ ．０５） 。结论 ｂｃｌ２ 表达与角质形成细胞角化不全有关，而 Ｆａｓ Ｌ 阴性提示 Ｆａｓ Ｆａｓ Ｌ途径介导的凋亡在银屑病角质形成细胞凋亡过程中不起主要作用。

Objective In order to study the abnormal proliferation and differentiation mechanisms of keratinocytes in psoriasis.Method The expression of bcl 2,Fas and FasL in lesions of psoriasis vulgaris was detected.Results In the lesional skin of psoriasis vulgaris,the bcl 2 was expressed in 7 cases and mainly localized in upper prikle cell layer and parakeratotic cells in horny layer.All the 10 cases expressed the Fas and the distribution of it was similar to bcl 2,the infiltraing inflammatory cells didn't express FasL.The correlation analysis showed that the expressive intensity of bcl 2 and Fas in keratinocytes was significantly negative(r=-0.707, P <0.05).Conclusion This suggests that bcl 2 is involved in the parakeratosis of keratinocytes,and Fas FasL process don't play major role in apoptosis of keratinocytes in psoriasis vulgaris.