γ-干扰素和地塞米松对成纤维细胞生长的影响

Effects of interferon and dexamathasone on growth of fibroblasts stimulated by interleukin1\

目的观察γ干扰素（ＩＦＮγ）和地塞米松（Ｄｅｘａ）对正常和ＩＬ１刺激的成纤维细胞生长的影响，旨在从细胞因子角度为抗纤维化有效措施的制定提供依据。方法用ＭＴＴ比色、ＡｇＮＯＲｓ银染和免疫组化技术，观察两种抑制剂对成纤维细胞增殖、ＡｇＮＯＲｓ合成和Ⅲ型胶原表达的影响。结果２５０×１０３Ｕ／Ｌ浓度的ＩＦＮγ和１００μｇ／Ｌ浓度的Ｄｅｘａ均能明显抑制正常和ＩＬ１刺激的成纤维细胞增殖、ＡｇＮＯＲｓ合成和Ⅲ型胶原表达。结论一定浓度的ＩＦＮγ和Ｄｅｘａ对正常和ＩＬ１刺激的成纤维细胞生长、增殖、ＡｇＮＯＲｓ合成和Ⅲ型胶原表达均显示出明显的抑制作用。

Objectives\ Our studies were aimed at providing a basis for effective therapeutic strategy against fibrosis using cytokines through observing the effects of interferon (IFN) and dexamathasone (dexa) on growth of normal and interleukin1(IL1)stimulated fibroblasts. Methods BZ]MTT colorimetry,a silver colloid technique to identify nucleolar organizer regions (AgNORs) and immunohistochemical methods were used to study the effects of the two inhibitors on growth of fibroblasts. Results Growth,proliferation activity,synthesis of AgNORs and expression of collagen type in normal and IL1stimulated fibroblasts were depressed evidently by IFN at 250103 U/L and dexa at 100g/L. Conclusions The authors believe that IFN and dexa at certain concentrations can inhibit obviously the growth, proliferation,AgNORs synthesis and collagen type expression of IL1stimulated fibroblasts.