rhTPO/GM-CSF融合蛋白对^(60)Co γ射线照射小鼠造血功能的影响

Influence of rhTPO/GMCSF fusion protein on hemopoiesis in mice irradiated with 60 Co rays\

目的探索一个新的、具有能同时促进机体红、粒及巨核细胞系造血的细胞因子，以便治疗由化疗和放疗等各种原因引起全血细胞损伤的综合征。方法所用的细胞因子是本实验室重组的产品，以照射小鼠为模型，给小鼠注射细胞因子后，观察红、粒及巨核细胞的变化。结果ｒｈＴＰＯ／ＧＭＣＳＦ融合蛋白对受照射小鼠血象有恢复作用。

Objective\ To find a new biological therapy for secondary hematopoietic failure including anemia,infection and hemorrhage after administration of chemotherapeutic drugs etc. Methods hGMCSF gene was ligated with hTPO gene isolated from human fetal liver mRNA and a new fusion protein rhTPO/GMCSF obtained. Results The new fusion protein could promote recovery of peripheral WBC and PLT of 5.0 Gy irradiated mice.BFUE,CFUMeg and CFUGM in bone marrow of mice after irradiation recovered significantly by treatment with rhTPO/GMCSF fusion protein for 10 days. Conclusion These results suggest that the new fusion protein has the biological activity of both hTPO and hGMCSF simultaneously and can stimulate the proliferation of megakaryocyte and granulocyte progenitors.