用差异显示法研究云锡矿工肺癌组织相关基因

Search for genes related to tin miners lung cancer tissues by differential display of mRNA\

目的为探讨矿工肺癌发生的机理，作者研究了与云锡矿工肺癌组织相关的基因。方法以云南锡矿工肺癌切除术的癌组织和正常肺组织为样品，用差异显示法比较两种组织差异表达基因，并将其克隆测序。结果在肺癌组织和同例正常肺组织中发现有３０个表达差异的基因片段。其中，１６个片段仅在肺癌组织中表达而不在正常组织中表达；１４个片段仅在正常组织中表达而不在肺癌组织中表达；测定了６个片段的序列。ＣＧ２、ＣＧ７、ＣＧ８、ＣＡ５和ＣＣ６５个序列在Ｇｅｎｂａｎｋ中未查到有７５％同源的序列，被认为是新序列并在Ｇｅｎｂａｎｋ中登录。ＣＧ３与人核糖体蛋白Ｌ２７ａ有９５％同源性。结论在矿工肺癌组织和同例正常肺组织中有已知和未知的表达差异基因存在。

Objective\ To search for the genes related to tin miners lung cancer tissues. Methods Differential display of mRNA.] Results Thirty cDNA fragments which differentially expressed in lung cancer tissues and the same patients normal lung tissues were discovered.Among these,16 expressed in lung cancer tissues,not in normal lung tissues;14 expressed on the contrary.Six cDNA fragment sequences were determined ,of which 5 sequences coded CG2,CG7,CG8,CA5 and CC6 had less than 75% of homology with known sequences in GenBank BLAST,so they were believed to be new sequences which we have recorded in Genbank.Only one fragment sequence coded CG3 had up to 95% of homology with human ribosome protein L27a gene. Conclusion mRNA differential display provides a unique and powerful experimental system to study differential gene expression in tin miners lung cancer tissues and the same patients normal lung tissues.Using the system,differential expression of 30 cDNA fragments was observed.Six of them may be used in study on molecular mechanism of miners radonassociated lung cancer.