鸡蛋白蛋白优选人精子

Optimization of human spermatozoa using chicken egg albumin

比较不同鸡蛋白蛋白（ Ｃ Ｅ Ａ）柱对 ３０ 份正常生育力精液进行精子优选的效果。分别以 ７５％单层 一步法（ Ｄ Ｃ）、７５％ 和１２５％ 双层一步法（ Ｓ Ｃ）及 ７５％ 预 选（ Ｆ Ｄ）再经 １０％ 、１５５％ 和 １７５％ 三层二步法（ Ｔ Ｃ） Ｃ Ｅ Ａ 柱进行优选。比较优选前后精子活动率、前向运动精子百分率和畸形率等常规指标及优选后各组精子回收率，并以盐酸喹啉染色比较优选前后精子 ｙ 小体阳性率。结果：优选后各组精子常规指标显著提高，以 Ｆ Ｄ 组精子回收率最高（３４７９％ ），高于上游法进行优选的回收率，与用人血清白蛋白进行优选的效果相当，可作为临床精子优选的有效手段。 Ｄ Ｃ、 Ｓ Ｃ 和 Ｔ Ｃ 各组回收率在 ２％ ～９％ 之间，显著低于 Ｆ Ｄ 组，但 ｙ 小体阳性 率均较优选前显著提 高， 在通过 Ｘ、 Ｙ 精子 分离以预防 Ｘ 连锁遗 传病上的可能用途有待进一步研究。

Objective: Several kinds of chicken egg albumin (CEA) gradients were used to enrich the human sperm with good routine index. The enriching efficiency of each column was compared. Methods: 30 semen samples from normal healthy volunteers were used for this experiment. Sperms were treated sequentially with 7.5% single column single layer (DC), 7.5% and 17.5% single column two layer (SC) and 10%, 15.5% and 17.5% two column three layer (TC) through preselecting (FD) in 7.5% CEA gradienls: X and Y bearing sperm were detected by the quinacrine 2HCL fluorecent staining method. Results: Post treatment with CEA column. The sperm motility, percentage of progressively motile sperm increased remarkably ( P <0.05). The percentage of abnormal sperm decreased ( P <0.05). 34 79% recovery of sperm in FD group was significantly higher than that in DC (below 10%) and that of SC and TC (below 5%)( P <0.05). The positive ratio of Y body in DC, SC, and TC increased remarkably ( P <0.05). Conclusion: CEA could be used in selection of good quality sperm. The sperm from FD were more favorable than those from DC, SC and TC. The high positive ratio of Y body in the later three groups suggested that these kinds of CEA gradients could be used to enrich Y bearing sperms. The value of CEA to prevent X linked genetic disease by enriching Y sperm remained to be studied futher.