摘要
目的:建立高效液相色谱法测定妇科十味片中阿魏酸的含量测定方法。方法:色谱柱为Zorbaxl C18流动相为乙腈-0.085%磷酸溶液(17:83),检测波长316nm,流速为1.0mL.min-1。结果:阿魏酸进样量在5.032~30.192μg.mL-1范围内线性关系良好(r=0.9998,n=6)。阿魏酸的平均回收率为98.76%,RSD为1.09%(n=6)。结论:本法简便、准确,重现性好,可作为妇科十味片的质量控制方法。
Objective : To establish a method for the determination of ferulie acid in Fukeshiwei Tablets by HPLC. Method : HPLC was used Zorbaxl C18 column,mobile phase was composed of Acetonitrile-0.085%H3PO4(17 : 83),The flow rate was 1.0mL·min-1 with detection wavelength at 316nm.Result : The linear range of ferulic acid was from 5.032 - 30.192 μg.mL-1 ( r =0.9998,n=6).The average recoverery was 98.76%,and RSD was 1.09%(n=6) Conclusion: The method is simple,accurate and suitable.It can be used For the quality control of Fukeshiwei Tablets.