人胎盘培养上清液对单克隆抗体分泌的影响

Influence of Human Placental Culture Supernatants on Antibody Production

本研究在培养分泌抗甲状腺球蛋白单克隆抗体（ Ｉｇ Ｇ 型） 杂交瘤 Ｈ４ 株时， 加入不同浓度（０ ％ 、２ ．５ ％ 、５ ％ 、１０ ％ 、２０ ％ ） 的人胎盘培养上清液（ Ｈ Ｐ Ｓ） ，培养不同的时间（２４ｈ 、４８ｈ 、７２ｈ 、９６ｈ）后取上清液，用 Ｅ Ｌ Ｉ Ｓ Ａ 法检测各培养上清液中抗体含量。结果表明， 低浓度（２ ．５ ％ 、５ ％ ） Ｈ Ｐ Ｓ 能够使 Ｈ４ 单抗分泌增强（ Ｐ＜ ０ ．０１） ；而高浓度（２０ ％ ） 的 Ｈ Ｐ Ｓ 能够使 Ｈ４ 单抗分泌减弱（ Ｐ＜ ０ ．０１） 。研究表明， Ｈ Ｐ Ｓ 在体外可作用于有亲代 Ｂ 细胞特性的杂交瘤细胞株 Ｈ４ ，对 Ｈ４ 株分泌单抗的量可由于 Ｈ Ｐ Ｓ 浓度不同而有促进或抑制作用， 以此探讨 Ｈ Ｐ Ｓ 对 Ｂ 细胞分泌抗体的影响， 以期阐明胎盘在保护胎儿中的作用机制。

Different concentrations(0%,2.5%,5%,10%,20%)of HPS (human placental culture supernatants) were added to IgG hybridoma culture (H 4) and antithyroid globulin protein antibody was collected from the supernatants after being cultured for 24h,48h,72h,96h to examine the antibody production by ELISA.The results showed low concentration (2.5%,5%)of HPS could increase the antibody production while high concentration(20%) of HPS can decrease it ( P <0.01).The observation indicated that HPS could affect the hybridoma H 4 which had the primary B cell character, and increased or decreased its antibody production depending on the concentration. Therefore, can it help us investigate the influence of HPS on antibody production of B cells and elucidate the mechanism that placenta plays a role in fetal protection.