CA242测定对恶性肿瘤的诊断价值

Application of CA242 IRMA in Clinical Detection

为研究肿瘤标志物 Ｃ Ａ２４２ 免疫放射分析的临床应用价值，应用１２５ Ｉ标记糖类抗原 Ｃ２４２的单克隆抗体 Ｃ Ａ２４２ ，固相珠用单克隆抗体 Ｃ２４１ 包被，建立夹心的免疫放射分析（ Ｉ Ｒ Ｍ Ａ） 。结果表明，１００ 名正常人和５３ 例良性疾病患者，其 Ｃ Ａ２４２ 分别为５６７ ±５８１ Ｕ／ ｍ Ｌ 和６８０ ±４１３ Ｕ／ｍ Ｌ。如果以１７ Ｕ／ｍ Ｌ 为阳性界值测定１６７ 例恶性肿瘤患者，其阳性率为６８３ ％ ；其中胰腺癌、结－ 直肠癌、胃癌和肺癌分别有８００ ％ 、５９７ ％ 、７００ ％ 和６００ ％ 的阳性率，而正常人的假阳性率为４０ ％ 。还对 Ｃ Ａ２４２ 的两种测定方法（ Ｉ Ｒ Ｍ Ａ 和 Ｅ Ｉ Ａ） 做了相关性研究，相关系数（ ｒ） 为０９６ 。同时还应用 Ｒ Ｏ Ｃ 曲线论证了 Ｃ Ａ２４２ 的临床应用和价值。结论是： Ｃ Ａ２４２ 在恶性肿瘤诊断方面有较高的灵敏度和特异性，是一种理想的肿瘤标志物。

The object is to research the application value of the tumor marker CA242 immunoradiometric assay(IRMA)in the clinical detection.Using Mab C241 against glycoprotein antigen CA242 as the capturing antibody and Mab C242 as the labeled antibody, we have developed a“sandwich”IRMA. In Clinical detection,of all the 100 healthy,53 benign disease and 167 malignant disease serum samples,the mean concentrations of the CA 242 are 5 67±5 81U/mL,6.80±4.13U/mL and 144 69±247 04U/mL,respectively. The mean concentration of the malignant diseases is much higher than that of the healthy samples and benign disease samples.If 17U/mL is used as the cut-off value,the positive rates of the pancreatic cancer and colorectal cancer are 80.0% and 59.7%,respectively. While the false positive rate in healthy samples is 4%.In this report we ont only have made an experiment on the relativity of IRMA and EIA ( r is 0.96),but also have discussed the value of ROC curve assay in the application of CA242 IRMA in clinical cancer detection.. CA242 has higher sensitivity and specificity in the diagnosis of the malignant disease.It is an ideal tumor marker.