抗凝剂、温度和性别对体外中性粒细胞自发性激活的影响

Effects of Anticoagulants, Temperature and Sex on the Spontaneous Activation of Neutrophils in Vitro

从细胞形态学和细胞酶组织化学的角度出发，用伪足法和ＮＢＴ 法检测体外中性粒细胞自发性激活，研究了抗凝剂、孵育温度和性别对检测结果的影响。发现：（１）ＥＤＴＡ 可显著抑制中性粒细胞自发性激活，血标本以小剂量肝素抗凝为宜；（２）孵育温度对中性粒细胞自发性激活有影响，３７ ℃比２５ ℃的激活百分率高，最高激活百分率出现较２５ ℃早１ ｈ；（３）女性中性粒细胞自发性激活百分率较男性高，最高激活百分率出现较男性早１ ｈ。研究结果为以后深入研究中性粒细胞激活与多种疾病的关系提供了参照系统。

From the view of cellular morphology and enzymic histochemistry, we used the pseudopod method and NBT test to measure spontaneous activation of neutrophils in vitro.The effects of anticoagulant,temperature, and sex on the percentage of spontaneously activated neutrophils were studied. The results suggest that heparin is a better anticoagulant than EDTA because EDTA inhibits neutrophil activation; that a higher percentage of activated neutrophils can be obtained when incubated at 37℃, compared with the percentage at 25℃, and at 37℃ the highest percentage appears an hour earlier; and that females show a higher percentage of spontaneously activated neutrophils than males, and females' highest percentage appears an hour earlier than males'. These are of referential value for further studies.