血清肝细胞生长因子与结直肠癌转移及预后的关系

Relationship of serum hepatocyte growth factor with metastasis and prognosis of colorectal cancer

目的探讨结直肠癌患者血清中肝细胞生长因子(HGF)的表达水平及其与结直肠癌转移和预后的关系,以及其作为结直肠肿瘤标志物的可行性。方法应用夹心ELISA方法检测65例结、直肠癌患者及20例健康志愿者血清HGF,癌胚抗原(CEA),糖抗原(CA19-9)的表达水平;Kap-lan-Meier法计算患者生存率;Cox生存分析血HGF,癌胚抗原(CEA),糖抗原(CA19-9)水平对患者预后的影响。结果结直肠癌淋巴结转移组HGF水平为(0.37±0.11)ng/mL;T3-4期为(0.32±0.29)ng/mL;肝转移组血清HGF水平为(0.43±0.10)ng/mL,上3组均显著高于对照组的(0.18±0.07)ng/mL(P<0.05),亦明显高于各对应组(P<0.05)。单因素分析显示HGF水平越高者生存期越短。结论血清HGF水平与结直肠癌发展及转移有密切关系;结直肠癌患者血HGF水平增高提示患者预后差。HGF可作为检测结、直肠癌转移和判断预后的临床参考指标。

Objective To investigate serum level of hepatocyte growth factor（HGF） in patients with colorectal cancer and its relationship with colorectal cancer metastasis and prognosis,as well as its potential role being used as a tumor marker for colorectal cancer.Methods Serum levels of HGF,carcinoembryonic antigen（CEA） and carbohydrate antigen（CA19-9） were determined in 65 cases of colorectal cancer and 20 cases of healthy volunteers by ELISA assay.Survival rates were caculated by Kaplan-Meier method.Survival analysis of each measured prognostic factor was conducted by Cox proportional hazard model.Results The serum HGF level in colorectal cancer patients with lymph nodes metastasis,T3-4 stage tumor and liver metastasis was（0.37±0.11）,（0.32±0.29） and（0.43±0.10） ng/mL respectively,and each was significantly higher than that in healthy control [（0.18±0.07） ng/mL,（P0.05）].Univariate analysis showed HGF levels significantly correlated with survival rate in colorectal cancer,and the higher the HGF level,the lower the survival rate.Conclusions The serum HGF level is significantly associated with the development and metastasis of colorectal cancer,an elevated HGF level indicates a poor prognosis.Serum HGF potentially be used as a clinical indicator to predict metastasis and prognosis of colorectal cancer.