多孔磷酸三钙陶瓷骨内植入后的超微结构研究

ULTRASTRUCTURE INVESTIGATION OF POROUS TRICALCIUM PHOSPHATE CERAMICS AFTER IMPLANTATION INTO RABBIT FEMUR

本文报道采用偏光显微镜和扫描电镜 Ｘ 射线能谱研究了多孔磷酸三钙陶瓷（β－ Ｔ Ｃ Ｐ） 骨内植入后的超微结构。从实验上说明了多孔磷酸三钙陶瓷的生物降解和新骨生成过程，为探讨可降解磷酸三钙陶瓷的生物降解和骨生成机理提供了证据。在体液和活细胞的作用下，磷酸三钙发生溶解，离解为磷酸根离子，通过细胞的活化作用以及碱性磷酸酶、磷酸化酶、 Ａ Ｔ Ｐ 酶、焦磷酸酶等多种生物酶的生物催化作用，使无机磷酸根参与一系列生命代谢的生化反应进行生物转化，不仅本身能作为骨生长的支架而且能逐渐降解被吸收，引导新骨生成并参与宿主骨的生命过程是可降解磷酸三钙多孔材料不同于其他材料的独特之处。

Ultrastructure of host bone tissue and biodegradable porous tricalcium phosphate (β-TCP) ceramics implants were studied using polarizing microscopy and scan electron microscopy EDXA 45 days after implantation into femoral condyle cavities of rabbits. The results showed the process of biodegradation and bone formation of the β-TCP materials. The ultrastructure provided evidence for understanding biodegradation and bone bonding mechanisms. These results demonstrated that macrophages and osteoclast took part in degradation of β-TCP ceramics by phagocytosis and extracellular resorption. Due to the presence of osteoblasts and osteocytes, the osteogenesis was completed. Moreover, a family of phosphorus dependent and calcium dependent enzymes were thought to play catalysis and active role in the biochemical reaction of bio tranformation of β-TCP material, so the β-TCP porous material could take part in new bone formation of host, thus showing its unique advantage.