人喉癌组织中支原体感染的检测及其临床意义

The expression of PD4 and it's significance in human laryngeal carcinoma

目的:探讨支原体单克隆抗体PD4在人喉癌不同组织中的表达及其临床意义。方法:采用免疫组织化学Envision法对256例喉不同病变组织标本(喉癌105例,颈部转移淋巴结31例,喉癌前病变18例,声带息肉53例,癌旁正常组织20例,癌周正常组织29例)进行PD4表达检测。结果:①人喉癌组织中PD4高表达率为44.76%(47/105),明显高于喉其他不同病变组织(P<0.01或P<0.05)。②中晚期(Ⅲ-Ⅳ期)、伴颈淋巴结转移、分化程度低(G3、G2)的喉癌病变组织中PD4表达阳性率明显高于早期(Ⅰ-Ⅱ期)、无颈淋巴结转移、分化程度高(G1)的喉癌组织(P<0.01或P<0.05)。③PD4表达阳性病例3、5年生存率明显低于PD4表达阴性病例(均P<0.01)。结论:喉癌的发生、发展及预后与支原体感染密切相关。

Objective：To explore the expression of mycoplasma monoclonal antibody PD4 in different laryngeal carcinoma tissues and their clinical significance.Method：Using immunohistochemistry methods to detect 256 cases of laryngeal tissues,including 105 cases of laryngeal carcinoma tissues,31 cases of neck metastasis lymph node tissues,18 cases of precarcinoma tissues,53 cases of vocal cord polyps,20 cases of normal tissues adjacent to laryngeal carcinoma and 29 cases of normal tissues around laryngeal carcinoma.Result：①The positive rate of PD4 was 44.76% in laryngeal carcinoma,which was much higher than that in other laryngeal tissues（P0.01 or P0.05）.②The PD4 expression in laryngeal carcinoma was higher in the third and fourth stages than in the first and second stages,higher with cervical metastasis than those without cervical metastasis and higher in the third and second histological grade than in the first histological grade（P0.01 or P0.05）.③To 3 and 5 years survival,PD4 positive cases had lower chance than PD4 negative cases（P0.01）.Conclusion：These results implicate that the occurrence,development and prognosis of laryngeal carcinoma may be closely related to mycoplasma infection.