丙烯酰胺对雄性小鼠生殖毒性的研究

The study on the reproductive toxicity of acrylamide in male mice

目的评价丙烯酰胺(acrylamide,AA)对睾丸细胞DNA损伤及精子核成熟率的影响,为研究AA生殖系统损伤机制提供实验室数据。方法以20、40和60 mg/kg AA经口灌胃染毒雄性小鼠,并设对照组,连续给药5 d,首次给药后14 d时,用彗星实验和吖啶橙荧光染色法检测睾丸细胞DNA损伤和小鼠精子核成熟率。结果彗星实验结果显示:60 mg/kg AA组中各监测指标与对照组比较,差异均有统计学意义(P<0.01),且随AA剂量的增加,各指标均明显增高,有显著的剂量-效应关系。吖啶橙荧光染色法结果显示:40和60 mg/kg剂量组小鼠精子核成熟率与对照组比较,差异有统计学意义(P<0.01),且随着染毒剂量的增加,精子核成熟率逐渐下降。结论 AA对小鼠睾丸细胞DNA有持续损伤作用,并与染毒剂量明显相关。AA破坏精子核DNA的完整性,导致精子核成熟率降低,以致于精子受精能力降低。

Objective To Evaluate the effects of acrylamide on DNA damage in the testicular cells and sperm cellular maturity which could provides laboratory data for studying the mechanism of damge to reproductive system by AA.Methods The male mice were randomly divided into 4 groups,the control group was given H2 O,while the experimental groups were given AA by mouth at dosages of 20、 40、60mg/kg for five successive days.The mice were sacrificed on the 14th day after the first AA treatment.SCGE and acridine orange fluorescent staining were used to assess the AA-induced DNA single strand breakage in mice testicular cells and cellular maturity sperm.Results From the comet assay,60mg/kg AA group showed statistical significance compared with control group（P 0.01）.The indicators were significantly higher along with the AA increased,and significant dose-response relationship of AA-induced DNA lesion to testicle cell（P 0.01）.From the acridine orange fluorescent staining assay,40 and 60mg/kg AA groups showed statistical significance compared with control group（P 0.01）.Sperm cellular maturity was declined along with the AA increased.Conclusion AA had sustained damage on mice testicular cells DNA which were correlated with the dose of the AA.AA undermined the integrity of the sperm DNA,which result in the decline of the sperm cellular maturity.