摘要
目的:研究蛋氨酸-胆碱缺乏(MCD)饮食诱导的非酒精性脂肪性肝炎(NASH)小鼠肝脏脂肪变性及炎症发生、发展的病理特点.方法:将40只C57/BL6小鼠随机分成2组,分别喂养MCD饮食(模型组)和对照蛋氨酸-胆碱充足(MCS)饮食(空白组),于造模的第1-5周动态连续取材,小鼠血清ALT、AST、TG及肝TG等生化指标通过全自动生化分析仪进行测定,肝脏标本通过油红O、HE染色对脂肪变性及炎症进行评分.结果:MCD饮食喂养2-3wk后,肝脏病理切片可见脂肪变及炎症,小鼠血清ALT(U/L)、AST(U/L)明显升高,与空白组相比有统计学差异(133.9±6.3vs47.3±1.3;154.3±24.6vs109.2±50.4,均P<0.05).在第3周时,模型组血清TG(mmol/L)含量较空白组明显降低(0.97±0.14vs1.45±0.32,P<0.01),而在第5周时,模型组肝TG(mmol/L)含量较空白组明显升高(1.50±0.22vs0.98±0.16,P<0.01).肝脏炎症坏死以腺泡1区为主为重,5wk时肝内纤维化表现开始出现.结论:MCD饮食诱导的NASH小鼠模型重复率高,造模2-3wk时模型稳定性最好.
AIM:To establish a mouse model of non-alcoholic steatohepatitis(NASH)by feeding a methionine-choline-deficient(MCD)diet and to dynamically monitor the pathological features of hepatic steatosis and inflammation in these mice.METHODS:Forty mice were randomly divided into two groups and fed an MCD diet or a me-thionine-choline-sufficient(MCS)diet.At weeks 1 to 5,these mice were sacrificed to take serum samples for biochemical measurements and liver samples for HE staining and oil red O staining to grade the degree of fatty change and inflam-mation.RESULTS:Steatosis and inflammation could be seen in mice fed an MCD diet for 2-3 wk.Serum ALT(U/L),AST(U/L)levels increased signifi-cantly(133.9±6.3 vs 47.3±1.3;154.3±24.6 vs 109.2±50.4,both P0.05),whereas TG(mmol/L) levels decreased(0.97±0.14 vs 1.45±0.32,P0.01)at week 3 in rats fed an MCD diet than in those fed an MCS diet.At wk 5,TG(mmol/L) levels in the liver increased significantly in rats fed an MCD diet than in controls(1.50±0.22 vs 0.98±0.16,P0.01).Fibrosis could be seen at week 5 in mice fed an MCD diet.CONCLUSION:A mouse model of NASH has been created successfully by feeding an MCD diet.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第11期1122-1129,共8页
World Chinese Journal of Digestology
基金
2010年北京中医药大学自主选题基金资助项目
No.2009JYBZ2-XS070
2010年北京市自然科学基金资助项目
No.7102094~~