ANXA2、VEGF对HepG2肝癌细胞系转移的促进作用

ANXA-2 and VEGF promote invasiveness of human liver cancer HepG2 cells

目的:探讨膜联蛋白2(ANXA2)、血管内皮生长因子(VEGF)促进HepG2肝癌细胞系转移的作用.方法:应用Transwell、RT-PCR、Western blot,检测在不同5-氟尿嘧啶(5-FU)剂量(50、100、200、400mg/L)干扰下,ANXA2、VEGF的表达及其促进HepG2肝癌细胞系转移的作用.结果:Transwell方法检测HepG2肝癌细胞随着5-FU剂量的增加侵袭力较对照组明显降低,各剂量组间差异具有明显统计学意义(22±5,25±4,13±2,12±2vs39±7,均P<0.05);RT-PCR、Westernblot方法检测HepG2肝癌细胞中ANXA2、VEGFmRNA、蛋白的表达量随着5-FU剂量的增加逐渐下降,与对照组间的差异具有显著统计学意义(ANXA2mRNA:0.527±0.008,0.419±0.046,0.213±0.007,0.176±0.007vs0.718±0.008;ANXA2蛋白:0.669±0.055,0.484±0.072,0.180±0.034,0.099±0.009vs1.236±0.102;VEGFmRNA:0.818±0.016,0.558±0.101,0.386±0.009,0.352±0.017vs1.176±0.035;VEGF蛋白:0.960±0.085,0.962±0.056,0.376±0.069,0.219±0.008vs1.124±0.170,均P=0.000),且两者具有相关性(rp=0.900,rw=0.856).结论:随着5-FU剂量的逐渐增加,HepG2肝癌细胞侵袭率及ANXA2、VEGF表达量逐渐下降,两者呈显著正相关,提示两者在肝癌细胞的侵袭转移机制中可能起重要作用.

AIM：To investigate the role of annexin-2（ANXA-2）and vascular endothelial growth factor（VEGF）in promoting invasiveness of human liver cancer HepG2 cells.METHODS：After HepG2 cells were treated with different doses of 5-fluorouracil,cell invasiveness was detected by Transwell assay,and the mRNA and protein expression of ANXA-2 and VEGF was detected by RT-PCR and Western blot,respectively.RESULTS：The invasiveness of HepG2 cells decreased with the increase in the dose of 5-fluorouracil,with significant differences among cells treated with different doses of 5-fluorouracil（22 ±5,25±4,13±2,12±2 vs 39±7,all P0.05）.The mRNA and protein expression of ANXA2 and VEGF in HepG2 cells decreased graduallywith the increase in the dose of 5-fluorouracil（ANXA2 mRNA：0.527±0.008,0.419±0.046,0.213±0.007,0.176±0.007 vs 0.718±0.008;ANXA2 protein：0.669±0.055,0.484±0.072,0.180 ±0.034,0.099±0.009 vs 1.236±0.102;VEGF mRNA：0.818±0.016,0.558±0.101,0.386±0.009,0.352±0.017 vs 1.176±0.035;VEGF protein：0.960±0.085,0.962±0.056,0.376±0.069,0.219± 0.008 vs 1.124±0.170,all P0.001）.There were significant correlations between the mRNA and protein expression of ANXA2 and VEGF（rp= 0.900,rw=0.856）.CONCLUSION：The expression of ANXA2 and VEGF in HepG2 cells decreased gradually with the increase in the dose of 5-fluorouracil.ANXA2 and VEGF may play an important role in the invasion and metastasis of hepatocellular carcinoma.