摘要
目的 探讨促甲状腺激素（TSH）对3T3-L1脂肪细胞葡萄糖转运蛋白4（GLUT4）表达的影响。方法体外诱导3T3-L1前脂肪细胞为成熟脂肪细胞。以不同浓度牛TSH（0．01IU／L、0．1IU／L、1IU／L）刺激成熟脂肪细胞，蛋白质印迹法分别检测48h后成熟脂肪细胞GLUT4蛋白表达水平，ELISA方法检测细胞培养液中白细胞介素6（IL-6）水平。结果与空白对照组相比，以不同浓度（0．01、0．1、1IU／L）牛TSH刺激成熟脂肪细胞48h后GLUT4蛋白表达呈下降趋势，分别下降约23％、44％、74％（均P〈0．05），细胞培养液中IL-6水平明显增加[（101．9300±6．8396）、（104．1533±5．9619）、（129．01674-6．4232）μg／L比（93．2500±2．6695）μg／L，P〈0．05]。结论TSH可下调3T3-L1脂肪细胞GLUT4蛋白的表达。
Objective To test if thyroid-stimulating hormone （TSH） can suppress expression of glucose transporter 4（GLUT4） in 3T3-L1 adipocytes. Methods 3T3-L1 preadipocytes were induced to differentiate into adipocytes. These adipocytes were treated with 0 to 1.0 IU/L TSH for up to 48 hours. Protein levels of total GLUT4 were quantified by western blotting. Culture medium interleukin-6 level was assessed by enzyme-linked immunosorbent assay（ELISA）. Results The GLUT4 protein level after 48 hours induced with different dose（0. 01 IU/L, 0. 1 IU/L, 1 IU/L） of TSH presented a significant 23%, 44%, 74% （ all P 〈 0.05） reduction compared with control group. While the interleukin-6 level of culture medium increased significantly [ （ 101. 9300 ± 6. 8396 ）, （ 104. 1533 ±5. 9619）, （ 129. 0167± 6.4232）μg/L vs （93. 2500 ±2. 6695） μg/L, P 〈 0.05 ]. Conclusion In 3T3-L1 adipocytes, TSH can decrease expression of GLUT43.
出处
《中国医药》
2011年第6期674-676,共3页
China Medicine
基金
天津市应用基础及前沿技术研究计划（10JCZDJC19900）
