氯诺昔康静脉镇痛配伍药物含量的高效液相色谱法测定

Determination of ketamine, tramadol, butyibromide, droperidol, metamizole and Iornoxtcam in analgesic mixture samples by high performance liquid chromatography

目的 建立应用高效液相色谱（HPLC）技术同时测定静脉镇痛液中盐酸氯胺酮、盐酸曲马多、丁溴东莨菪碱、氟哌利多、咪达唑仑与氯诺昔康含量的方法。方法采用日本DL-C18（150mm×4．6mm，5μm）色谱柱，乙腈旬．05mol／L磷酸二氢钾水溶液（21：79）为流动相，流速为0．8ml／min。结果盐酸氯胺酮、盐酸曲马多、丁溴东莨菪碱、氟哌利多、咪达唑仑与氯诺昔康分别在40～400mg／L，8—800mg／L，0．5～100mg／L，0．5—25mg／L，4～80mg／L与4—400mg／L浓度范围与峰面积具有良好的线性关系；平均加样回收率分别为98．6％、101．1％、100．4％、99．5％、98．3％与99．8％；相对标准偏差分别为1．12％、0．67％、0．95％、1．23％、1．72％与0．78％。结论本方法方便、快速、准确，可以作为盐酸氯胺酮、盐酸曲马多、丁溴东莨菪碱、氟哌利多、咪达唑仑与氯诺昔康配伍检测的方法。

Objective To establish a high performance liquid chromatography（HPLC） method for determination of ketamine, tramadol, butylbromide, droperidol, metamizole and lornoxicam in analgesic mixture samples. Methods The chromatographic separation was achieved on DL-C18 （150 mm× 4.6 mm, 5 μm） with the mobile phase consisting of acetonitile and 0.05 mol/L potassium dihydrogen phosphate solution （21 ： 79） at a flow rate of 0.8 ml/min. Results The linearities were in the ranges of 40-400 mg/L for ketamine, 8-800 mg/L for tramadol, 0.5-100 mg/L for butylbromide ,0. 5-25 mg/L for droperidoi,4-80 mg/L for metamizole and 4-400 mg/L for lornoxicam. The average recovery was 98.6%, 101.1%, 100.4%, 99.5%, 98.3% and 99.8% ,respectively. RSD was 1.12%, 0.67%, 0.95%, 1.23%, 1.72% and 0.78%. Conclusion The method is sensitive,rapid and accurate and suitable for compatible determination of ketamine, tramadol, butylbromide,droperidol, metamizole and lornoxicam in analgesic mixture samples.