类风湿关节炎患者T细胞受体BV基因的表达

Expression of T cell receptor BV gene in rheumatoid arthritis patients

目的 检测中国人群中类风湿关节炎（ Ｒ Ａ） 患者 Ｔ 细胞 Ｔ Ｃ Ｒ Ｂ Ｖ 基因表达的偏移。方法 采用半定量 Ｒ Ｔ Ｐ Ｃ Ｒ 方法，分析６ 例 Ｒ Ａ 患者的外周血、关节液、滑膜（ 每例均取三种标本） 中新鲜分离的未经 Ｉ Ｌ２ 刺激的 Ｔ 细胞 Ｔ Ｃ Ｒ Ｂ Ｖ１ ～２０ 基因片段的表达。结果 患者外周血与正常人外周血 Ｔ Ｃ Ｒ Ｂ Ｖ 基因表达格局相似；患者关节液与滑膜 Ｔ Ｃ Ｒ Ｂ Ｖ 基因表达格局相似；而患者关节内（ 关节液＋ 滑膜） Ｔ Ｃ Ｒ Ｂ Ｖ 基因表达格局与外周血相比， Ｂ Ｖ２ 、 Ｂ Ｖ７ 、 Ｂ Ｖ１１ 高， Ｂ Ｖ１３１ 、 Ｂ Ｖ２０ 低。结论 Ｒ Ａ 患者关节内 Ｔ 细胞在对 Ｔ Ｃ Ｒ Ｂ Ｖ 基因片段的优势表达。

Objective To detect the biased expression of TCR BV gene in Chinese rheumatoid arthritis (RA) patients.Methods With the semi quantitative PCR method,the expression of TCR BV 1～20 gene elements of T cells which have not been activated by IL 2 was analysed.These T cells were freshly isolated from peripheral blood,synovial fluid and synovial membrane from six RA patients.Results There was no difference in BV gene expression pattern between PB T cell of RA patients and controls.The expression of TCR BV gene elments was identical in SF and SM T cells;but the BV2,BV7 and BV11 were increased former while the BV13 1 and BV20 were increased latter when the expression pattern was compared between SF+SM TCR BV gene and PB TCR BV gene of the RA patients.Conclusion There is a prior expression of TCR BV gene elements in the T cells in the arthrosis of RA patients.