单相酶扩散法测定SLE患者血清DNA酶的浓度

Measurement of serum deoxyribonuclease in patients with SLE by single radial enzyme diffusion method

目的 建立 Ｄ Ｎａｓｅ 检测的方法，并探讨血清 Ｄ Ｎａｓｅ 浓度异常在 Ｓ Ｌ Ｅ 发病中的意义。方法 于国内首次用单相酶扩散法检测了６３ 例 Ｓ Ｌ Ｅ 患者。结果 Ｓ Ｌ Ｅ 患者血清中 Ｄ Ｎａｓｅ 浓度明显低于正常人，活动期患者明显低于缓解期患者，血清 Ｄ Ｎａｓｅ 的浓度下降与 Ｄ Ｎ Ａ 含量升高呈负相关。用于 Ｄ Ｎａｓｅ 检测的琼脂板中最适 Ｄ Ｎ Ａ 和 Ｅ Ｂ 浓度分别为３０ ％ 和２５ ％ ，该法检测 Ｄ Ｎａｓｅ 的灵敏度可达２ μｇ／ Ｌ。结论 用单相酶扩散法检测血清 Ｄ Ｎａｓｅ 浓度简便、灵敏、可靠，血清 Ｄ Ｎａｓｅ 水平低下在 Ｓ Ｌ Ｅ 的发生发展中起着一定作用，测定患者 Ｄ Ｎａｓｅ 浓度在研究 Ｓ Ｌ Ｅ 复杂的免疫发病机制上具有重要意义。

Objective To establish the method detecting DNase and measure serum DNase in SLE patients.Methods The concentration of DNase was measured by single radial enzyme diffusion method in 63 serum samples of SLE patients.Results The concentration of DNase in SLE patients was significantly lower than that in healthy individuals.Patients at active stage showed much lower DNase level than patients at inactive stage,and decrease of serun DNase was negatively correlated with the increase of serum DNA.The optimal concentration of DNA and EB in agar plate was 30% and 2 5%,and the sensitivity detecting DNase was up to 2 μg/L.Conclusion The single radial enzyme diffusion method for measuring DNase is simple,sensitive and reliable.The decrease of serum DNase may play an important role in the development of SLE.