不同侵袭力癌细胞内质网膜系统的对比研究

Comparative investigation of endoplasmic reticulum in cancer cells with different invasive ability

目的：研究癌细胞的内质网膜系统与其侵袭性之间的相互关系。方法：应用共焦激光扫描显微技术观察不同侵袭力的人大肠癌细胞 Ｃ Ｃ Ｌ２２９ 及 Ｃ Ｘ１ 的内质网膜系统，结合流式细胞仪技术测定两者内质网膜系统的荧光强度。结果：两种癌细胞内均存在着立体网络状结构的内质网膜系统，细胞核周围致密，周边逐渐稀疏。高侵袭力的 Ｃ Ｃ Ｌ２２９ 细胞的内质网发达，伪足中存在独枝或立体网络状的内质网，而低侵袭力的 Ｃ Ｘ１ 细胞的内质网相对不发达，未见伪足样结构。流式细胞仪检测 Ｃ Ｃ Ｌ２２９ 细胞的 Ｅ Ｒ 的平均荧光强度为１ ０２４ ±５０２９７ ， Ｃ Ｘ１ 细胞的 Ｅ Ｒ 的平均荧光强度为４６２９０ ±２１７１８ ，统计学处理两者差异显著（ Ｐ＜ ００１） 。结论：高侵袭力的 Ｃ Ｃ Ｌ２２９ 细胞与低侵袭力的 Ｃ Ｘ１ 细胞的内质网膜系统形态及数量上均存在差异，可能与内质网合成和侵袭相关之分泌蛋白的功能有关。

Purpose To investigate the relationship between invasiveness and Endoplasmic Reticulum (ER). Methods Confocal laser scanning microscopy and flow cytometry were used to study the morphology and quantity of ER in human colorectal cancer cell lines CCL 229 and CX 1 which are of different in vasiveness. Results There were three dimentional network like ER in both CCL 229 and CX 1, which were mainly distributed around nucleus, and became disparse in the periphery of cell. There were more developed ER in CCL 229 than in CX 1. There was the single tubule of ER in spike like pseudopedia, and the comple ER in lambular psudopedia in CCL 229. While these structures were not found in CX 1. The average flurescene intensity of ER of CCL 229 was 1 024 ±502 97 while that of CX 1 was 462 90±217 18 according to flow cytometry results, which showed a great statistically difference statisic analysis ( P< 0 01). Conclusion The results suggest that there are differences in the morphology and quantity of ER betweens CCL 229 and CX 1, and the difference of ER which plays an important role in synthesis of protein relating to invasiveness may play a part in invisiveness.