孕鼠高血糖对胎鼠胰岛功能的影响

Influences of Gestational Hyperglycemia on the Function of Fetal Rat Pancreatic Islets

目的 探讨孕鼠高血糖对胎鼠胰岛β细胞分泌胰岛素功能的影响。方法 ２８ 只妊娠 Ｓ Ｄ 大鼠随机分成两组，实验组在妊娠晚期持续性静脉输注高渗葡萄糖；对照组同期输注无菌蒸馏水。采用胎鼠胰岛细胞进行胰岛素抽提实验、胰岛素释放试验及灌流试验，研究胎鼠胰岛β细胞功能的变化。结果 实验组胎鼠每个胰腺内胰岛素平均水平为（３ ３２９ ．８１ ±１６３ ．３９）ｎｇ；单位重量胰腺组织胰岛素水平为（１５８ ．５６ ±２３ ．３４）ｎｇ／ｍｇ。对照组胎鼠胰腺内胰岛素平均水平为（２ ３９０ ．０４ ±１５１ ．３９）ｎｇ ；单位重量胰腺组织胰岛素水平为（１２５ ．７９ ±１７ ．９７）ｎｇ／ｍｇ 。两者比较，差异有显著性（ Ｐ＜ ０ ．０５） 。在低糖或高糖 Ｈａｎｋｓ 液中温育胰腺组织２ 小时，实验组胎鼠胰岛素释放量：低糖时为（２４ ．２２ ±４ ．６３）ｎｇ／ｍｇ ；高糖时为（７３ ．７３ ±５ ．９４）ｎｇ／ｍｇ 。对照组胎鼠胰岛素释放量：低糖时为（２０ ．５２ ±２ ．３１）ｎｇ／ｍｇ ；高糖时为（５３ ．３５ ±７ ．５３）ｎｇ／ｍｇ 。两组比较，差异有显著性（ Ｐ＜ ０ ．０５） 。在胰岛素灌流试验中，实验组胎鼠胰岛素分泌时相高峰出现早且峰值高于对照组。

Objective To investigate the influences of gestational hyperglycemia on the function of fetal rat pancreatic islets. Methods Twenty eight pregnant SD rats were randomly allocated into two groups, The study group was continually infused with 30% glucose at the rate of 2ml/min, while the controls with sterilized distilled water in the same time and at the same rate. The infusion began on day 16.5 post coitus and lasted for 5 days until the removal of the fetuses. The functions of the β cells in fetal rat islets were studied by determining the content of insulin, using the tests of extracting, releasing and perfusing. Results The data showed that the insulin content of study group (3 329.81±163.39)ng was significantly higher than that of controls (2 390.04±151.39)ng ( P <0.05); After 2 hours′ incubation in Hanks solution which contained different concentration of glucose, increased insulin secretion was found in the study group ( P <0.05); When the islets were perfused, the insulin peak value of study group was higher and appeared earlier than that of controls. Conclusion Insulin synthesis and secretion function of β cells of fetal pancreas from hyperglycemia rats were enhanced and the pancreas had a hypersensitive response to glucose challenge.