GFP标记的TH基因在帕金森病大鼠脑内的表达

Expression of HTH_1 Genes Marked by GFP in Brains

目的:探讨HTH_1基因治疗帕金森病的可能性。方法:以绿色荧光蛋白(GFP)作为标记,观察HTH_1基因在体外和脑内的表达。结果:构建同时表达GFP和HTH_1双基因的多基因表达载体GC-GFP-P-HTH_1-SN,并转染L-6TG细胞,移植入帕金森病模型大鼠纹状体内,用荧光显微镜、流式细胞仪、免疫组化、Western blotting等方法检测GFP、THT_1在体外和脑内均有表达。结论:本实验为外源基因表达产物的检测提供一个新方法。

Aim: To explore possibility of treating Parkinson's disease by HTH1 gene. Methods: By using GFP gene as a marker, to observe expression of human tyrosine hydroxylase type I(HTH1)gene in vitro or in brains. Results: The multiple gene expression vector GC-GFP-P-HTH1-SN which contained both GFP cDNA and HTH1 cDNA was constructed and transfected into L-6TG cells. L-6TG cells which co-expressed GFP and HTH1 were then implanted into the striatum of PD rats. The expression of HTH1 in vitro or in brain was detected by fluorescent microscope, flow cytometry, immunohistochemical staining and Western blotting,etc. Conclusion: This study was designed to provide a new method to observe expression of exogenous gene.