摘要
目的观察转化生长因子-β（TGFβ-1）在博莱霉素（BLM）所诱导的肺纤维化大鼠肺泡灌洗液中的动态变化及其意义。方法 BLM制成大鼠肺间质纤维化模型,观察组13只大鼠气管注入BLM 0.2~0.3m l,对照组5只大鼠气管注入等量生理盐水,并将各组动物处死后提取肺组织及支气管肺泡灌洗液（BALF）进行病理定量分析,检测BALF中TGFβ-1的水平变化。结果观察组TGFβ-1水平高于对照组,第7、14天比较差异有统计学意义（P〈0.01）。TGFβ-1在观察组成纤维细胞（Fb）和肺泡巨噬细胞（AM）中表达于实验第7天显著增强,于实验第14天达到高峰,至第28天有所降低,但明显高于对照组（P〈0.05或P〈0.01）。结论 TGFβ-1在肺纤维化中及其病理发生发展过程中均发挥重要作用。
Objective To investigate the gene expression of TGF-β in bleomycin（BLM） induced in mice with pulmonary fibrosis in alveolar lavage fluid. Methods BLM made model of pulmonary fibrosis in rats,observation group（ 13 rats） injected BIM O. 2 -0.3ml in trachea,control group（5 rats） injected with normal saline,and animals in 2 groups were sacrificed after the extraction of lung tissue and bronchoalveolar lavage fluid （BALF） for pathological quantitative analysis of detection of the level of TGF-β1 in BALF. Results The level of TGF-β in observation group was higher than that in control group, compared the level of the 7,14d,the difference was statistically significant（ P 〈 O. 01 ）. The expression of TGF-[31 of the fibroblast（Fb） and alveolar macrophages（AM） in observation group increased on the 7th day, the 14th day in the experimental peak,the 28th day decreased,but significantly higher than that in control group（ P 〈 0.05 or P 〈 0.01 ）. Conclusion TGF-β in the pathogenesis of pulmonary fibrosis and development play an important role.
出处
《临床合理用药杂志》
2011年第9期29-30,共2页
Chinese Journal of Clinical Rational Drug Use
