中药石斛对大鼠胃热证的作用研究

Effect study of dendrobium in traditional Chinese medicine on stomach-heat syndrome in rats

目的探讨中药石斛对胃热证大鼠全身体征及胃部生理机能的影响。方法 120只SD大鼠随机分为空白对照组1、空白对照组2、模型对照组1、模型对照组2、黄连大剂量组、黄连小剂量组、石斛大剂量组、石斛小剂量组各15只。用1.0kg/L的干姜以5mg/kg剂量灌胃大鼠连续15d制成胃热证大鼠模型,对石斛大、小剂量组应用不同剂量进行治疗,对黄连大、小剂量组应用不同剂量进行治疗,观察石斛大、小剂量对大鼠全身体征及胃部生理机能的影响。结果造模后模型对照组1、模型对照组2、黄连大剂量组、黄连小剂量组、石斛大剂量组、石斛小剂量组大鼠日平均食量高于造模前,差异均有统计学意义(P<0.05);治疗后黄连大剂量组、黄连小剂量组、石斛大剂量组、石斛小剂量组大鼠日平均食量低于造模后,差异均有统计学意义(P<0.05)。造模后模型对照组1、模型对照组2、黄连大剂量组、黄连小剂量组、石斛大剂量组、石斛小剂量组大鼠日平均尿量低于造模前,差异均有统计学意义(P<0.05);治疗后黄连大剂量组、黄连小剂量组、石斛大剂量组、石斛小剂量组大鼠日平均尿量高于造模后,差异均有统计学意义(P<0.05)。模型对照组大鼠血浆中6-Keto-PGF1α含量低于空白对照组,差异有统计学意义(P<0.05),但TXB2较空白对照组升高不明显,差异无统计学意义(P>0.05),TXB2/6-Keto-PGF1α较空白对照组升高,差异有统计学意义(P<0.05)。治疗后黄连大、小剂量组和石斛大、小剂量组大鼠血浆中6-Keto-PGF1α含量明显高于模型对照组,TXB2和TXB2/6-Keto-PGF1α明显低于模型对照组,差异均有统计学意义(P<0.05)。石斛大、小剂量组TXB2含量和TXB2/6-Keto-PGF1α均高于黄连大、小剂量组,6-Keto-PGF1α含量低于黄连大、小剂量组,差异均有统计学意义(P<0.05)。结论石斛对大鼠胃热证具有治疗作用。

Objective To explore the effect of dendrobium on the systemic sign and gastric physiological function of rat with stomach-heat syndrome.Methods 120 SD rats were randomly divided into the blank control group1,blank control group2,model control group1,model control group2,large-dose coptis group,low-dose coptis group,large-dose dendrobium group and low-dose dendrobium group,15 rats of each.1.0kg/L of dried ginger was fed to rats for 15 days by a dose of 5mg/kg in order to make the model of rats with stomach-heat syndrome.The treatment was instituted with different doses in large-dose and low-dose dendrobium group,and the treatment was instituted with different doses in large-dose and low-dose coptis group.Observed the effects of large-dose and low-dose dendrobium on the systemic sign and gastric physiological function in rats.Results After making model the average feed daily of the model control group1,model control group2,large-dose coptis group,low-dose coptis group,large-dose dendrobium group and low-dose dendrobium group were higher than those of the 6 groups before making model,and the differences were statistically significant （P0.05）;After treatment the average feed daily of the large-dose coptis group,low-dose coptis group,large-dose dendrobium group and low-dose dendrobium group were lower than those of the 4 groups after making model,and the differences were statistically significant （P0.05）.After making model the rats＇ average urinary output daily of the model control group 1,model control group 2,large-dose coptis group,low-dose coptis group,large-dose dendrobium group and low-dose dendrobium group were lower than those of the 6 groups before making model,and the differences were statistically significant （P0.05）;After treatment the rats＇ average urinary output daily of the large-dose coptis group,low-dose coptis group,large-dose dendrobium and low-dose dendrobium were higher than those of the 4 groups after making model,and the differences were statistically significant （P0.05）.The plasma content of 6-keto-PGF1α in model control group was lower than that in bland control group,and the difference was statistically significant （P0.05）,but the plasma content of TXB2 in model control group was not sensible increased compared with blank control group,so there was no statistical significance between 2 groups （P0.05）;and the TXB2/6-keto-PGF1α in model control group was higher than that in blank control group,and the difference was statistically significant （P0.05）.After treatment,the plasma content of 6-keto-PGF1α in large-dose coptis group and low-dose coptis group were higher than those in model control group;And the TXB2,TXB2/6-keto-PGF1α in large-dose coptis group and low-dose coptis group were lower than those in model control group,and the differences were statistically significant （P0.05）.The TXB2 and TXB2/6-keto-PGF1α in large-dose dendrobium group and low-dose dendrobium group were higher than those in large-dose coptis group and low-dose coptis group,but the 6-keto-PGF1α in which was lower than that in large-dose coptis group and low-dose coptis group,and the differences were statistically significant （P0.05）.Conclusion Dendrobium was effective in the treatment of stomach-heat syndrome of rats.