感冒止咳颗粒质量标准研究

Study on quality standard of Ganmaozhike Granule

目的建立感冒止咳颗粒的质量标准。方法用薄层色谱(TLC)法对桔梗、前胡、陈皮、枳壳进行定性鉴别;用高效液相色谱(HPLC)法测定陈皮、枳壳中橙皮苷和柚皮苷的含量。结果 TLC图斑点清晰,阴性对照无干扰;橙皮苷在0.1880～0.9400μg范围内线性关系良好,平均回收率为98.77%,RSD为0.83%(n=6),柚皮苷在0.1868～0.9340μg范围内线性关系良好,平均回收率为98.37%,RSD为1.26%(n=6)。结论该方法专属性强,灵敏度高,重现性好,可用于感冒止咳颗粒的质量控制。

Objective To establish the quality standard of Ganmaozhike Granule.Methods Radix platycodonis,radix peucedani,dried tangerine peel and fructus aurantii were identified by thin layer chromatography（TLC）;The content of hespiridin and naringin in fructus aurantii and dried tangerine peel were determined by high performance liquid chromatography（HPLC）.Results The TLC spots were fairly clear,and the negative control showed no interference.The calibration curve of Hespiridin was linear in the range of 0.1880～0.9400μg,its average recovery was 98.77% and RSD was 0.83%（n=6）.The calibration curve of Naringin was linear in the range of 0.1868～0.9340μg,its average recovery was 98.37% and RSD was 1.26%（n=6）.Conclusion The specificity,sensitivity and reproducibility of the method are good,which can be used for quality control of Ganmaozhike Granule.