HIV-1感染者外周血Ⅰ型干扰素产生细胞水平变化的研究

Changes on type Ⅰ IFN-producing cell level in patients with human immunodeficiency virus-1 infection

目的探讨我国HIV感染者外周血Ⅰ型干扰素产生细胞(IPC)水平及其与疾病进展的关系。方法应用流式细胞术以全血染色、全白细胞设门对92例HIV-1感染者和59例健康对照进行IPC水平的测定,并分析外周血IPC变化与CD4+T细胞计数和HIV血浆病毒载量的关系。结果 HIV感染者外周血CD4+T细胞及IPC绝对计数的均数分别为342.000个/μl和3.431个/μl,显著低于正常对照(965.000个/μl和5.995个/μl,P均<0.001);HIV感染者IPC细胞数量与CD4+T细胞计数成正比(r=0.430,P<0.001),与HIV血浆病毒载量成反比(r=-0.483,P<0.001);CD4+T淋巴细胞计数<200个/μl的感染者IPC水平明显低于CD4+T淋巴细胞计数>200个/μl者(P<0.005)。HIV慢性感染者的IPC水平显著高于艾滋病患者(P<0.001),新发感染者的IPC水平(5.080个/μl)明显低于正常对照(P=0.038),但新发感染与慢性进展者的IPC水平间的差异无统计学意义。结论 HIV感染可显著降低机体的IPC水平,IPC水平变化与HIV疾病进展密切相关。

Objective To investigate the profiles of the type Ⅰ IFN-producing cell（IPC）level in patients with HIV-1 infection in different disease status.Methods Fifty-nine HIV-1 negative controls and ninety-two subjects infected with HIV-1 were enrolled in this study.The absolute numbers of IPCs were measured by flow cytometry through whole blood dyeing and whole white cells gating.Association between IPCs and CD4＋ T cells and viral loads were analyzed.Results The median cell counts of CD4＋ T lymphocytes（342.000 cells/μl）and IPCs（3.431 cells/μl）in HIV infected subjects were both significantly lower than that of the controls（P〈0.001）.The absolute counts of IPCs in patients with HIV-1 infection correlated positively with CD4＋ T cells（r=0.430,P〈0.001）,while negatively with viral load（r =-0.483,P〈0.001）.When stratifying the HIV-1 infected subjects with CD4＋ T cell counts,individuals with CD4＋ T cell counts below 200 cells/μl have significantly less IPCs than that of subjects with CD4＋ T cell counts above 200 cells/μl（P〈0.005）.The median count of IPCs in patients with AIDS was lower than that of HIV chronic progressors and primary infectors（P〈 0.001）,while there was no statistical difference between HIV chronic progressors and primary infectors.Conclusions The absolute counts of IPCs in HIV-1 infected subjects were dramatically decreased and correlated with disease progression.