维生素D受体基因与儿童急性白血病遗传易感性的关系研究

Study on relationship between ApaI site polymorphism of vitamin D receptor gene and hereditary susceptibility to childhood acute leukemia

目的探讨维生素D受体(VDR)基因ApaI位点多态性与儿童急性白血病遗传易感性的关系。方法采用聚合酶链限制性片段长度多态性技术和基因测序技术,对127例急性白血病患儿和268例健康儿童的VDR基因ApaI位点多态性进行分析。结果 VDR基因ApaI酶切位点基因型和等位基因的频率在急性白血病患儿和正常儿童之间差异无统计学意义。结论 VDR基因ApaI酶切位点的多态性可能与儿童急性白血病的遗传易感性无关。

Objective To investigate the relationship between ApaI site gene polymorphism of vitamin D receptor （VDR） and hereditary susceptibility to acute childhood leukemia. Methods Polymerase chain reaction- restrictive fragment length polymorphism analysis technique and DNA sequencing technology were used to detect polymorphisms of VDR gene ApaI locus in 127 children with acute leukemia and 268 healthy children （as controls）. Results No significant differences in genotype distribution frequencies and the allele gene distribution frequencies of VDR gene ApaI locus were found between the two groups. Conclusions The gene polymorphisms of VDR gene ApaI enzyme digestion locus may not be related to genetic susceptibility to acute childhood leukemia.