丹参对大鼠液压脑损伤后神经生长因子表达的调节作用

Danshen injection affecting NGF positive cells after traumatic brain injury in rats by fluid percussion

目的探讨丹参对大鼠脑损伤后神经生长因子（NGF）的表达是否有促进作用；脑损伤后神经生长因子水平与时间的关系。方法SD大鼠72只，分成三组（假手术组、脑损伤组及丹参治疗组），采用液压脑损伤装置建立侧方液压打击脑损伤模型，利用免疫组织化学SABC法显示脑损伤后不同时相点神经生长因子的表达及丹参处理后对神经生长因子表达的影响。结果当发生脑损伤后，脑损伤组脑组织NGF的表达量3d后开始升高（1．29±0．61），7d后达高峰（2．03±0．42），14d后则降至一般水平（0．87±0．23）。丹参治疗组脑组织NGF的表达早期较假手术组有明显增加（1．77±0．54，3．35±0．37，0．65±0．21VS0．75±0．31，1．58±0．52，0．49±0．36，P〈0．05～0．01），丹参治疗组14d后的水平低于脑损伤组（0．65±O．21VS0．87±0．23，P〈0．05）。结论脑损伤后脑组织中NGF表达量显著升高，并呈现一定的规律性，这对于探索脑损伤时间的推断方法具有重要的实验研究意义。丹参注射液能促进脑外伤后神经因子的表达，对脑损伤有保护作用。

Objective To study on NGF positive cells changes by the action of Danshen and ex plore the correlation between time and amount of NGF after traumatic brain injury （TBI）in rat. Methods 72 rats were randomly divided into 3 groups, including sham group, brain injury and Danshen therapy group. TBI rat models were made by fluid percussion. NGF was determined on the 3rd, 7th and 14th day after TBI. Results NGF positive cells after traumatic brain injury in brain reached the maximum level at the 3rd day（ 1.29±0. 61 ）, and it decreased since the 7th day（2. 03 ±0. 42）, and it decreased to the lowest level on the 14th day （0. 87 ± O. 23 ）. NGF positive cells after traumatic brain injury treated by Danshen were more than those patients who were treated without Danshen（ 1.77 ±0. 54,3. 35 ＋0. 37,0. 65 ± 0. 21 vs 0.75±0.31,1.58±0.52,0.49±0.36, P 〈0.05±0.01）. Conclusions The expression of NGF was significantly increased in brain damaged tissues. This may be served as a useful marker of diagnosis in timecourse of brain leison. Danshen injection can be used to protect brain after traumatic brain injury.