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牛布鲁氏菌病阳性血清国家标准品的研制 被引量:2

Preparation and characterization of follwing the national standard anti-Brucella abortus serum, bovine
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摘要 为制备标定凝集试验用的牛布鲁氏菌病阳性血清国家标准品,采集4份田间自然感染牛布鲁氏菌病的阳性血清作为候选物,经过筛选后,选择了3#血清用于标准品制备。对该标准品进行了物理性状、无菌检验、真空度测定、剩余水分检验、均匀性检测和稳定性试验,检测结果均符合要求。以国际标准品为参比,测定了该标准品的RBT、SAT和CFT效价,结果分别为1∶160"+"、1∶2 400"++"、1∶800"++",与协作标定结果完全一致。该标准品以国际标准品溯源的国际单位含量为4 000 IU/mL。 To prepare anti-Brucella abortus serum used for calibrate the agglutination test follwing the national standard,4 anti-Brucella abortus sera were obtained from 4 cows infected with Brucella abortus naturally.By potency testing,the third serum was selected.Sterility,vaccum degree,residual moisture,uniformity and stability of this standard material were tested and proved to meet the national standard.Referring to the international standard,RBT(Rose-Bengal plate agglutination test),SAT(standard tube agglutination) and CFT(complement fixation test) titers of this standard material were measured to be 1:160 "+",1:2 400 "++" and 1:800 "++",which are identical with the collaborative assay results.International unit of the standard material is 4 000 IU/mL.
出处 《生物工程学报》 CAS CSCD 北大核心 2011年第5期812-816,共5页 Chinese Journal of Biotechnology
基金 "十一五"国家科技支撑计划(No.2006BAD06A10)资助~~
关键词 牛布鲁氏菌病 阳性血清 国家标准品 效价测定 均匀性 稳定性 Brucella abortus anti-serum the national standard potency standardization homogeneity stability
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  • 1史新涛,古少鹏,郑明学,马海利.布鲁氏菌病的流行及防控研究概况[J].中国畜牧兽医,2010,37(3):204-207. 被引量:44
  • 2Davis DS, Elzer PH. Brucella vaccines in wildlife. Vet Microbiol, 2002, 90(1/4): 533-544.
  • 3Morgan WJB, MacKinnon DJ, Lawson JR, et al. The rose bengal plate agglutination test in the diagnosis of brucellosis. Vet Rec, 1969, 85(23): 636-641.
  • 4Nicoletti P. An evaluation of serologic tests used to diagnose brucellosis in buffaloes (Bubalus bubalis). Trop Anim Health Prod, 1992, 24(1): 40-44.
  • 5Mateu-de-Antonio EM, Martin M, Casal J. Comparison of serologic tests used in canine brucellosis diagnosis. J Vet Diagn Invest, 1994, 6(2): 257-259.
  • 6宋翠平,徐辉,王志亮,毕道荣.检测布氏杆菌病的四种血清学方法比较及国际标准单位的应用[J].中国动物检疫,2003,20(2):25-27. 被引量:12
  • 7WHO Expert Committee on Biological Standardization. World Health Organization Technical Report Series, No. 86. Geneva: World Health Organization, 1953.
  • 8WHO Expert Committee on Biological Standardization. World Health Organization Technical Report Series, No. 329. Geneva: World Health Organization, 1966.
  • 9农业部第四届兽用生物制品规程委员会.中华人民共和国兽用生物制品规程.2000年版.北京:化学工业出版社,2000.
  • 10世界动物卫生组织著.农业部兽医局/中同动物卫生与流行病学中心译.陆生动物诊断试验和疫苗手册(哺乳动物、禽鸟与蜜蜂).5版.巴黎:国际兽医局.2007.

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