桔梗花粉萌发与花粉管生长研究

Pollen Germination and Growth of Platycodon grandiflorum(Jacq.)A.DC

以2年生桔梗植株为材料,采用液体培养法研究了培养基种类、PEG、蔗糖、pH以及培养温度、培养时间对桔梗花粉离体萌发生长的影响,结果表明：（1）浓度为100～150 g.L-1的PEG可显著促进桔梗花粉萌发和花粉管的生长;200～250 g.L-1PEG显著促进花粉萌发,但对花粉管生长的作用不显著。（2）100 g.L-1的蔗糖有利于花粉萌发和花粉管生长,高浓度蔗糖（200 g.L-1）有明显抑制作用;（3）桔梗花粉离体萌发和花粉管生长的适宜培养基为ME3＋BK＋10%蔗糖＋150 g.L-1PEG（pH5.8）;（4）25～40℃条件下桔梗花粉均可较好萌发,以30℃培养1.5 h为最佳培养条件。

Two-year-old Platycodon grandiflorum（Jacq.）A.DC trees grown in the experiment field of Xiangju Pharmacy Limited Company were used as the materials.The effects of culture medium,sucrose,PEG,pH value,culture time and temperature on the pollen germination and tube growth of P.grandiflorum were investigated using the method of liquid culture.The results showed that：（1）the concentration of 100-150g·L-1 PEG could accelerate pollen germination and the growth of pollen tube,the range of 200-250g·L-1 PEG could remarkably accelerate pollen germination,however,its function on the growth of pollen tube was not distinct.（2）100g·L-1 sucrose was helpful for the pollens to germinate and grow,however,high concentration of sucrose can would strongly inhibit pollen germination and the growth of pollen tubes.（3）the optimum culture medium for pollen germination and growth of Platycodon were ME3＋BK＋10% sucrose＋150g·L-1 PEG（pH 5.8）.（4）pollen germination was better at 25-40℃,and it was best at 30℃ for 1.5h.