培养基对不同性别水蕨配子体叶绿素荧光特性的影响

Chlorophyll Fluorescence Analysis of Hermaphrodite and Male Gametophytes from Ceratopteris thalictroides(L.) Brongn.on Different Media

研究了MS和改良knop’s培养基条件下水蕨(Ceratopteris thalictroides)两性配子体和雄配子体叶绿素荧光参数的差异,结果表明:MS培养基中两性配子体PSⅡ线性电子传递的量子效率ФPSⅡ和PSⅡ驱动的电子传递速率ETRPSⅡ值显著高于MS培养基中的雄配子体以及改良knop’s培养基中的配子体;各组配子体光化学效率Fv/Fm值无显著差异;外源营养对各组配子体光化学荧光猝灭系数qP和非光化学荧光猝灭系数qN的影响不具明显规律性。综上所述,MS培养基中外源糖类的添加使水蕨配子体PSⅡ的光合活性增强,有利于大量快速生长的两性配子体的形成。

The differences of fluorescence parameters of hermaphrodite gametophyte and male gametophyte from Ceratopteris thalictroides cultured in MS medium and modified knop＇s medium were investigated.The results showed that the ФPSⅡ and ETRPSⅡ of hermaphrodite gametophyte in MS medium were higher than those of the male gametophyte in MS medium and gametophytes in modified knop＇s medium,the differences were significant.The values of Fv/Fm had no significant difference in the gametophytes of different groups.The external nutrient had no obvious effect on the qP and qN in the gametophytes of different teams.In conclusion,external sucrose enhanced photosynthetic activity of the gametophytes in MS medium,which was beneficial for hermaphrodite gametophytes to grow and develop rapidly.