利用烟草表达人源性胰岛素样生长因子1

Producing IGF-1 in Transgenic Tobacco

构建了由花椰菜花叶病毒35S启动子引导人源性胰岛素样生长因子1基因(igf-1)的表达载体pCAM-BIA1301-35S promoter-igf-1-nos,并利用根癌农杆菌LAB4404介导,将其导入烟草。经潮霉素抗性筛选、GUS检测和PCR鉴定,获得17棵转基因植株。RT-PCR分析结果显示,igf-1能够在转基因烟草中正常转录。本试验为利用烟草以及其他双子叶植物高效表达便于分离纯化的IGF-1药用蛋白研究奠定了重要的基础。

In this research,a new expression vector of pCAMBIA1301-35S promoter-igf-1-nos,which included the gene of hIGF-1,was constructed.The gene is promoted by CaMV 35S promoter which can be expressed in tobacco in high performance.The expression vector of IGF-1 cDNA introduced by 35S promoter was transformed into LBA4404（Agrobacterium tumefacients）,and then infected tobacco through leaf-disc infection.17 transgenic tobacco plants were gained after hygromycin selection,GUS test and PCR test of DNA.It shows IGF-1 gene can be transcribed into normal mRNA in transgenic tobacco by RT-PCR test.In this experiment,the expression of IGF-1 in tobacco lays the foundation for producing IGF-1 in tobacco and other dicotyledon bio-reactors as well.