翅果油树不同部位多糖含量及抗氧化活性比较研究

Comparisons of the Content and Antioxidant Activity of Polysaccharide from Different Parts of Elaeagnus mollis Diels

采用苯酚—硫酸法,测定比较了翅果油树叶片、枝条和种子3个部位的多糖含量;并分别采用邻苯三酚自氧化法和番红花红O—Mn2+—H2O2光度法测定比较了3个部位多糖对超氧离子、羟基自由基的清除活性。结果表明:翅果油树叶片、枝条和种子多糖平均含量依次为69.42,23.15,56.47 mg.g-1;3个部位多糖对羟基自由基、超氧阴离子自由基均有清除作用,其清除超氧离子自由基的活性依次为:Vc>种子>枝条>叶片,清除羟基自由基的活性依次为:叶片>种子>Vc>枝条。该研究结果表明,翅果油树叶片和种子均可作为利用该植物开发天然抗氧化剂的优选部位。

The content of polysaccharide from leaves,branches and seeds of endangered plants Elaeagnus mollis Diels was determined by the phenol-sulfuric acid method under 490 nm.The oxygen anion radical and hydroxyl radical scavenging activity of polysaccharide from three parts of E.mollis was studied by the pyrogallol self-oxidation method and spectrophotometry with safranine O—Mn2＋—H2O2 system.The results showed that the average levels of polysaccharide from leaves,branches and seeds of E.mollis were 69.42,23.15 and 56.47mg·g-1,respectively.The polysaccharide of three parts showed antioxidant activities in removing superoxide free radicals and hydroxyl radical.The scavenging activity on superoxide free radicals was Vcseedsbranchesleaves,removing hydroxyl radical activity was leavesseedsVcbranches.The research results showed that the leaves and seeds of E.mollis could be the raw material as natural antioxidant.