铜胁迫对苋菜叶片叶绿素a荧光诱导动力学的影响

Effects of Copper Stress on the Chlorophyll a Fluorescence Induction Kinetics of Amaranthus mangostanus L.

以苋菜(Amaranthus mangostanus L.)为材料,采用营养液中添加铜的培养方法,观测了根部生长变化;运用叶绿素荧光分析技术研究了铜胁迫下苋菜光系统Ⅱ(PSⅡ)反应中心结构和功能的变化以及PSⅡ反应中心供体侧和受体侧氧化还原状态的影响。结果表明:铜胁迫使苋菜根部生长受到抑制,叶片PSⅡ反应中心失活;最大光化学效率(FV/FM)下降;叶片PSⅡ受体侧产生严重的伤害,线性电子传递受阻;PSⅡ供体侧的放氧复合体(OEC)受损;铜胁迫对苋菜叶片PSⅡ的伤害从PSⅡ供体侧和受体侧开始。

The effect of Cu-added nutrient fluid on the root of Amaranthus mangostanus L.was investigated in this experiment.Also,the function changes of the photosystemⅡ（PSⅡ） reaction center in Amaranthus mangostanus L.,and the influences of copper in donor and acceptor sides of this system were investigated by the method of fluorescence induction kinetics.The results showed that copper stress inactivated the PSⅡreaction center in the leaves of Amaranthus mangostanus L.,decreased the maximal photochemical efficiency of PSⅡ（FV/FM）,and damaged the acceptor side of PSⅡseriously and inhibited the transfer of linear electrons,and also did harm to the oxygen evolving complexes（OEC） of the donor side of PSⅡ.It could be concluded that the damage of copper stress in Amaranthus mangostanus L.was begun from the donor and acceptor sides of PSⅡin leaves.