灵发素(LFS)在甘蔗组培中的应用

Application of LFS in Sugarcane Tissue Culture for Rapid Propagation

探讨LFS在甘蔗组培快繁中的作用与使用前景。以新台糖22号茎尖或其组培继代苗为试验材料,以MS为基本培养基,分别添加不同浓度的LFS,并以6-BA为诱导丛生芽及增殖对照,NAA为诱导生根对照,然后按常规方法进行诱导茎尖分化丛生芽及其增殖和生根等培养。结果显示:①LFS比6-BA显著减轻茎尖褐化,提高茎尖成活率,促进其分化丛生芽和增殖,并提高苗的质量。诱导茎尖分化丛生芽培养基中LFS的适宜浓度为2.5～3.0 mg/L;继代增殖的适宜浓度为3.0～5.0 mg/L,培养3代后,增殖倍数8.76倍,略高于CK的8.53倍,且苗长势旺,黄叶少;②LFS可诱导不定根的发生,适宜浓度在2.0～4.0 mg/L,发根率≥90%。表明LFS在甘蔗组培效果优于传统使用的6-BA,具有良好的应用前景。

A new hormone was used for sugarcane tissue culture and rapid propagation.The stem apexes of ROC22 and its sub-cultured plantlets were used as the materials.MS basal medium supplied with different concentrations of LFS as the treatment,while 6-BA as the control in the differentiating and multiplication stage,and NAA as the control in the rooting stage,respectively.It was revealed that LFS could significantly retard the browning of the stem apexes and increase stem apexes＇ survival ratio.It also demonstrated that 2.5～3.0 mg/L LFS was suitable for differentiation,while 3.0～5.0 mg/L for multiplication,and 8.76 times of multiplication rate was got after three subcultures while 8.53 times for the control.Compared with NAA,LFS could induce the occurrence of adventitious roots and the appropriate concentrations were between 2.0～4.0 mg/L with the rooting rate more than 90%.LFS showed favorable application prospects in sugarcane tissue culture for rapid propagation.