BTH诱导橡胶树对白粉病的抗性效果和相关酶活性测定

Effects of Rubber Tree Resistance Induced by BTH to Oidium heveae and Assay of Resistance-related Enzymes

选用几种抗性诱导剂处理橡胶树古铜期叶片,再接种橡胶树白粉菌,以研究诱导橡胶树对白粉病产生系统性抗性的能力,并测定有效药剂BTH(苯并噻二唑)处理后,诱导间隔期及叶片抗性相关酶活性变化。结果表明,用250 mg/L BTH喷施,5 d后接种白粉病,诱导抗性效果最佳,防效达57.8%。用BTH处理并接种病原菌后,POD和PAL酶活性与对照相比显著升高,可持续到15 d,并且再次施药,酶活性再次上升。这说明BTH可以诱导橡胶树对白粉病产生系统获得抗性。在生产中,橡胶树白粉病发病前,利用BTH诱导抗病性,并结合实际情况使用常规防治,可有效减少橡胶树白粉病的发生和危害。

In this experiment, the induced resistance on rubber tree to Oidium heveae was investigated by bronzecoloured leaves of rubber budding seedlings treated with several resistance inducers and then inoculated the leaves with Oidium heveae. The period of induced interval and resistance-related enzyme activity of leaves after BTH treatments were determined. The results showed that a better concentration of BTH induced resistance on rubber tree to Oidium heveae was 250 mg/L, up to 57.8% control and the best period of induced interval was 5 days after BTH treatment. The activities of phenylalanine ammonia lyase （PAL） and peroxidase （POD） increased significantly compared with the control after rubber tree leaves treated with BTH and then inoculated with Oidium heveae. This indicated that BTH could induce the systemic resistance on rubber tree to Oidium heveae. BTH induced disease resistance and combined with general control methods should be reduced effectively Oidium heveae infestation and decreased economic losses before Oidium heveae occurrence in rubber production.