摘要
采用响应曲面分析法对葡萄籽原花青素的提取工艺进行优化。对提取率影响因素进行单因素分析后,以Box-Behnken设计(BBD)评价提取剂浓度、料液比、提取时间和提取温度4个因素显著性和交互作用的分析,得出葡萄籽原花青素的最佳提取条件为:丙酮浓度75%,料液比1∶10(w/v),时间44 min,温度51℃。在该条件下,葡萄籽原花青素提取率的预测值为2.90%,验证为2.88%,纯度为55.92%。
The extraction technology of proanthocyanidins from grape seeds was studied using response Surface Method（RSM）,and acetone concentration,solid-liquid ratio,temperature and time were selected as experimental the factors.Based on the analysis of significance and interaction among different factors,the optimal condition of extraction technology was obtained as followed： acetone concentration was 75%,solid-liquid ratio was 1 ∶ 10,time was 44 min and temperature was 51 ℃,and the predicted value and measured value of proanthocyanidins yield was 2.90% and 2.88%,and the purity was 55.92%.
出处
《热带作物学报》
CSCD
2011年第3期554-559,共6页
Chinese Journal of Tropical Crops
基金
福建省科技创新平台建设项目(2007S1001)
福建省科技厅重大专项专题项目(2008NZ0001-4)
国家转基因重大专项重点课题(2009ZX08001-032B)
农业部948计划项目(2009-Z43)
福建省科技合作计划重点项目(2010I0001)
关键词
葡萄籽
原花青素
提取
响应曲面分析法
Grape seed
Proanthocyanidins
Extraction
Response Surface Method
