白芍提取物活性成分芍药苷大鼠在体肠吸收动力学的研究

The study in situ on rat intestinal absorption kinetics of the active component paeoniflorin in extract of raidix paeoniae alba

目的：考察芍药苷的大鼠在体肠吸收动力学性质。方法：采用大鼠在体单向灌流法,利用HPLC法测定芍药苷的含量,分别研究吸收部位、药物浓度、灌流速度对芍药苷吸收的影响。结果：各肠段间药物的吸收速率常数Ka和表观吸收系数Papp比较差异均无统计学意义（P〉0.05）;芍药苷浓度在5.52~21.43 mg/L范围内,吸收速率常数Ka和表观吸收系数Papp比较差异均无统计学意义（P〉0.05）;随着灌流速度的增加,吸收速率常数Ka和表观吸收系数Papp均明显增加。结论：芍药苷在全肠道均有吸收,且无特定吸收部位;吸收无高浓度饱和现象,提示芍药苷吸收主要为被动扩散机制。

Objective： To study the situ intestinal absorption kinetics of Paeoniflorin in rats.Methods： In situ single-pass perfusion model was used and the concentrations of the perfusate were determined by HPLC.Results： By the testing of the statistics,the Ka and the Papp values of the duodenum,jejunum,ileum,colonic had no significant differences（P0.05）.The concentration from 5.52-21.43 mg/L had no distinctive effect on the Ka and the Papp.When the flow rate was raised,the Ka and the Papp was increased significantly（P0.05）.Conclusion： Paeoniflorin could be absorbed at all segments of intestine in rats and no special absorption window is observed;The saturate absorption phenomena was not observed,and suggested that Paeoniflorin is absorbed by passive diffusion mechanism.