血管内皮细胞生长因子在胃癌中的表达及其临床意义

目的研究血管生长因子(VEGF)在胃癌发生、发展中的作用。方法应用逆转录-聚合酶链反应(RT-PCR)及计算机条带分析半定量方法检测VEGF在56例胃癌组织及相应癌周组织的表达水平。结果①胃癌中VEGF阳性表达率为87.75%(49/56),高于癌边组织中表达阳性率53.3%(16/30),(P<0.05);②胃癌中VEGF阳性表达率在不同分化程度组比较无显著性差异(P>0.05)。结论 VEGF在胃癌中高表达,提示其可能与胃癌的恶性进展有关,检测VEGF在胃癌中的表达对判断胃癌的恶性程度有价值。

Objective This study was to evaluate the relationship between VEGF expression and gastric cancer.Methods The expression of VEGF mRNA in 56 gastric carcinoma was examined by reverse transcription polymerase chain reaction（RT-PCR）.Results VEGF was found in cytoplastic and cell surface of most gastric carcinoma cell（positive staining 49 in 56）,the rate of positive staining in gastric carcinoma was the highest（P0.05）.Conclusion The expression of VEGF was high in gastric cancer,which indicate that high expression of VEGF may correlate with the malignancy of gastric cancer.