动脉粥样硬化模型大鼠ADP与TNF-α表达水平相关性研究

Research on the relevance between expression level of ADPN and TNF-α in rats of atherosclerosis

[目的]探讨脂联素(ADP)与肿瘤坏死因子-α(TNF-α)表达水平的相关性。[方法]选取wistar大鼠216只随机分为正常组、模型组、通脉化浊汤高剂量、中剂量、低剂量、中药对照组,以高脂饮食造模,给予不同干预措施,4、6、8周后分别处死12只大鼠,采用RT-PCR技术检测心肌细胞中ADP及TNF-α的表达水平。[结果]ADP与TNF-α的表达成负相关,且对TNF-α表达具有抑制作用。[结论]ADP在动脉粥样硬化过程中具有重要的调控及治疗作用。通脉化浊汤可提高ADP水平、抑制TNF-α的表达,控制和治疗动脉粥样硬化发生、发展,具有重要的临床意义及广阔的应用前景。

[Objective]To explore the relevance between expression level of adiponectin（ADPN） and tumor necrosis factor α（TNF-α）.[Methods]216 wistar rats were randomly divided into six groups： control group,model group,Tōng Mài Huà Zhuó Tāng high dose group,Tōng Mài Huà Zhuó Tāng middle dose group,Tōng Mài Huà Zhuó Tāng low dose group,and Chinese medicinals control group.The atherosclerosis model was induced by feeding the rats with cholesterol-rich fodder.Then the medicinals were administered on these rats,12 of which were executed in the fourth,sixth and eighth weeks counted from the day of administration.After the relevant tissues for research were taken,the expression levels of ADP and TNF-αin myocardial cell were tested using the technology of RT-PCR.[Results]There is a positive relevance between expression level of ADPN and TNF α,and also there is an inhibitory effect of ADP on TNF-α.Above all,recipes of Chinese medicine have clear regulatory effects on the expression of ADP.[Conclusion]Tōng Mài Huà Zhuó Tāng is very promising both clinically and practically in its effect on controlling and treating atherosclerosis by promoting the level of ADP that inhibits the expression of TNF-α.