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应用PCR-DGGE技术快速检测病原微生物的研究 被引量：5

The research of rapid detection of pathogenic microorganism by PCR-DGGE technology
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摘要 目的:建立临床病原微生物的快速检测方法。方法:采用PCR-DGGE技术,PCR扩增标准菌株和检验样品的16S rDNA中的V3可变区序列,通过变性梯度凝胶电泳(DGGE)技术来进行检测,并将电泳检测结果同传统分离培养结果进行对比。结果:PCR-DGGE技术检测样品结果同传统分离培养结果一致。结论:应用PCR-DGGE技术检测病原微生物,快速高效可行。 Objective：To explore the fast detection methods of pathogenic microorganism.Methods： Use the PCR-DGGE technology to amplify the V3 variable region of 16S rDNA of stand strains and simples,and detect by the denaturation gradient gel electrophoresis（DGGE） technology.Results： The detection results by PCR-DGGE technology are similar with the traditional isolating culture results.Conclusion： The detection methods of pathogenic microorganism using PCR-DGGE technology is fast and efficient.
作者 徐晓东 张孝卫 杜雪 王兵 李铁军 孔健
机构地区 济南市卫生科技交流服务中心 山东大学微生物国家重点实验室
出处 《中国卫生检验杂志》 CAS 2011年第5期1058-1060,共3页 Chinese Journal of Health Laboratory Technology
关键词 PCR-DGGE技术 病原微生物 快速检测 PCR-DGGE technology Pathogenic microorganism Rapid detection
分类号 R446.5 [医药卫生—诊断学]
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参考文献9

  • 1Fischer SG, Lerman LS. Length - independent separation of DNA restriction fragments in two - dimensional gel electrophoresis [ J ]. Cell, 1979, 16(1): 191-200.
  • 2Muyzer G, Dewaal EC, Uitterlinden AG. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction amplified genes coding for 16S rRNA [ J ]. Appl Environ Microbial, 1993, 59(3) : 695 -700.
  • 3Muyzer G. , K. Smalla. Application of denaturing gradient gel electro- phoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology[ J ]. Anotonie Van Leeuwenhoek, 1998, 73(1) : 127 -141.
  • 4Ercolini D. PCR- DGGE fingerprinting: novel strategies for detection of microbes in food[J]. J Microbiol Methods, 2004, 56 (3) : 297 - 314.
  • 5Fasoli S, Marzotto M, Rizzotti L. Bacterial composition of commercial probiotic products as evaluated by PCR- DGGE analysis[J]. International Journal of Food Microbiology, 2003,82 ( 1 ) : 59 - 70.
  • 6Teamxerman R, Scheirlinck I, Huys G. Cuhure - independent analysis of probiotics products by denaturing gradient gel electrophoresis [ J ]. Appl Environ Microbiol, 2003,69( 1 ) : 220 -226.
  • 7Randazzo CL, Toiriani S, Akkermans AD. Diversity, dynamics, and activity of bacterial communities during production of an artisanal sicilian cheese as evaluated by 16S rRNA analysis[J]. Appl Environ Microbiol, 2002,68(4) : 1882 - 1892.
  • 8冯薇,王加启,刘开朗,卜登攀,李旦,赵圣国,杨开伦.运用PCR-DGGE分析比较瘤胃中不同饲料固相粘附微生物区系[J].畜牧兽医学报,2010,41(12):1556-1562. 被引量：12
  • 9王福金,西野直树,王靖宇.应用PCR-DGGE技术对发酵全混合日粮中可培养乳酸菌的调查[J].动物营养学报,2010,22(6):1636-1643. 被引量：7

二级参考文献30

  • 1王小芬,王伟东,高丽娟,崔宗均.变性梯度凝胶电泳在环境微生物研究中的应用详解[J].中国农业大学学报,2006,11(5):1-7. 被引量：48
  • 2内田仙二.サイレージ研究の進展と課題[M] ∥内田仙二.サイレージ科学の進歩,東京:デーリィ■ジャパン,1999:24-32.
  • 3NISHINO N,HARADA H,SAKAGUCHI E.Evaluation of fermentation and aerobic stability of wet brewer's grains ensiled alone or in combination with various feeds as a total mixed ration[J].Journal of the Science of Food and Agriculture,2003,83:557-563.
  • 4NISHINO N,WADA H,YOSHIDA M,et al.Microbial counts,fermentation products,and aerobic stability of whole crop corn and a total mixed ration ensiled with and without inoculation of Lactobacillus casei or Lactobacillus buchneri[J].Journal of Dairy Science.2004,87:2563-2570.
  • 5NISHINO N,HATTORI H.Resistance to aerobic deterioration of total mixed ration silage inoculated with and without homofermentative or heterofermentative lactic acid bacteria[J].Journal of the Science of Food and Agriculture,2007,87:2420-2426.
  • 6WANG F J,NISHINO N.Resistance to aerobic deterioration of total mixed ration silage:effect of ration formulation,air infiltration and storage period on fermentation characteristics and aerobic stability[J].Journal of the Science of Food and Agriculture,2008,88:133-140.
  • 7増子孝義.サイレージの発酵[M] ∥内田仙二.サイレージ科学の進歩,東京:デーリィ,1999:86-89.
  • 8NISHINO N,YOSHIDA M,SHIOTA H,et al.Accumulation of 1,2-propanediol and enhancement of aerobic stability in whole crop maize silage inoculated with Lactobacillus bucheri[J].Journal of Applied Microbiology,2003,94:800-807.
  • 9NISHINO N,WADA H,YOSHIDA M,et al.Microbial counts,fermentation products,and aerobic stability of whole crop corn and a total mixed ration ensiled with and without inoculation of Lactobacillus casei or Lactobacillus buchneri[J].Journal of Dairy Science,2004,87:2563-2570.
  • 10WANG F J,NISHINO N.Ensiling of soybean curd residue and wet brewers grains with or without other feeds as a total mixed ration[J].Journal of Dairy Science,2008,91:2380-2387.

共引文献17

同被引文献65

  • 1吴清平,周艳红,蔡芷荷.卫生微生物特异性显色培养基的研究与应用[J].中国卫生检验杂志,2005,15(1):124-126. 被引量：72
  • 2万波,赵海,李安明,刘晓风,刘克鑫.冬尖发酵的微生物学研究[J].应用与环境生物学报,1995,1(2):196-200. 被引量：3
  • 3杨怀德,张才军,李秀义,王玲.流式细胞术在微生物学中的应用[J].医学综述,2006,12(13):825-827. 被引量：15
  • 4MUYZER G, de WAAL E C, UITTERLINDEN A G. Profilingof complex microbial populations by denaturing gradient gelelectrophoresis analysis of polymerase chain reaction-amplified genescoding for 16S rRNA[J]. Applied and Environmental Microbiology,1993, 59(3): 695-700.
  • 5ZOI P, NICHOLAS C, GWEN F, et al. Comparison of the bacterialspecies diversity of spontaneous cocoa bean fermentations carried outat selected farms in Ivory Coast and Brazil[J]. Food Microbiology,2011, 28(5): 964-973. DOI:10.1016/j.fm.2011.01.010.
  • 6GONZáLEZ-ARENZANA L, LóPEZ R, SANTAMARíA P, et al.Dynamics of lactic acid bacteria populations in Rioja wines by PCRDGGE,comparison with culture-dependent methods[J]. AppliedMicrobiology and Biotechnology, 2013, 97(15): 6931-6941.DOI:10.1007/s00253-013-4974-y.
  • 7NIKOLIC M, TERZIC-VIDOJEVIC A, JOVCIC B, et al .Characterization of lactic acid bacteria isolated from Bukuljac,a homemade goat’s milk cheese[J]. International Journal ofFood Microbiology, 2008, 122(1/2): 162-170. DOI:10.1016/j.ijfoodmicro.2007.11.075.
  • 8RANDAZZO C L, VAUGHAN E E, CAGGIA C. Artisanal andexperimental Pecorino Siciliano cheese: microbial dynamics duringmanufacture assessed by culturing and PCR-DGGE analyses[J].International Journal of Food Microbiology, 2006, 109(1/2): 1-8.DOI:10.1016/j.ijfoodmicro.2005.11.002.
  • 9RANDAZZO C L, RIBBERA A, PITINO I, et al. Diversity of bacterialpopulation of table olives assessed by PCR-DGGE analysis[J]. FoodMicrobiology, 2012, 32(1): 87-96. DOI:10.1016/j.fm.2012.04.013.
  • 10MARI M, YASUYUKI S, DONG H H, et al. Lactobacillus harbinensissp. nov., consisted of strains isolated from traditional fermentedvegetables ‘Suan cai’ in Harbin, Northeastern China and Lactobacillusperolens DSM 12745[J]. Systematic and Applied Microbiology, 2005,28(8): 688-694. DOI:10.1016/j.syapm.2005.04.001.

引证文献5

二级引证文献59

中国卫生检验杂志
2011年 第5期

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