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应用PCR-DGGE技术快速检测病原微生物的研究 被引量：5

The research of rapid detection of pathogenic microorganism by PCR-DGGE technology
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摘要 目的:建立临床病原微生物的快速检测方法。方法:采用PCR-DGGE技术,PCR扩增标准菌株和检验样品的16S rDNA中的V3可变区序列,通过变性梯度凝胶电泳(DGGE)技术来进行检测,并将电泳检测结果同传统分离培养结果进行对比。结果:PCR-DGGE技术检测样品结果同传统分离培养结果一致。结论:应用PCR-DGGE技术检测病原微生物,快速高效可行。 Objective：To explore the fast detection methods of pathogenic microorganism.Methods： Use the PCR-DGGE technology to amplify the V3 variable region of 16S rDNA of stand strains and simples,and detect by the denaturation gradient gel electrophoresis（DGGE） technology.Results： The detection results by PCR-DGGE technology are similar with the traditional isolating culture results.Conclusion： The detection methods of pathogenic microorganism using PCR-DGGE technology is fast and efficient.
作者 徐晓东 张孝卫 杜雪 王兵 李铁军 孔健
机构地区 济南市卫生科技交流服务中心 山东大学微生物国家重点实验室
出处 《中国卫生检验杂志》 CAS 2011年第5期1058-1060,共3页 Chinese Journal of Health Laboratory Technology
关键词 PCR-DGGE技术 病原微生物 快速检测 PCR-DGGE technology Pathogenic microorganism Rapid detection
分类号 R446.5 [医药卫生—诊断学]
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参考文献9

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共引文献17

同被引文献65

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引证文献5

二级引证文献60

中国卫生检验杂志
2011年 第5期

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