HPLC法测定八正合剂中芦荟大黄素、大黄酸、大黄素和大黄酚

Determination of aloeemodin,rhein,emodin and chrysophanol in Bazheng mixture by HPLC

目的:建立高效液相色谱法同时测定八正合剂中芦荟大黄素、大黄酸、大黄素和大黄酚的含量。方法:采用Lu-na-C18(150 mm×4.6 mm,5μm);流动相:甲醇-0.1%磷酸(80:20);流速:0.8 ml/min;检测波长:254 nm;柱温:35℃。结果:芦荟大黄素、大黄酸、大黄素和大黄酚的线性范围分别为0.032μg^0.324μg、0.039μg^0.387μg、0.0104μg^0.104μg和0.0103μg^0.103μg,相关系数均为0.9999;平均加样回收率分别为98.3%、98.7%、97.7%和97.2%;RSD分别为0.9%、0.8%、1.0%和1.1%(n=6)。结论:本法专属性好,准确度、重现性好,可作为制剂的定量分析方法。

Objective：To establish a method to determine the contents of aloeemodin,rhein,emodin and chrysophanol in Bazheng Mixture by HPLC.Methods： The samples were separated at 35℃ using a Luna-C18 column（150 mm×4.6 mm,5 μm）;The mobile phase consisted of methanol–phosphoric acid（80∶ 20）;at a flow rate of 0.8 ml/min;the wavelength of detection was 254 nm.Results： The calibration curves showed good linearity in the range of 0.032 μg^0.324 μg（aloeemodin）,0.039 μg^0.387 μg（rhein）,0.0104 μg^0.104 μg（emodin）,0.0103 μg^0.103 μg（chrysophanol）.The average recoveries were 98.3%,98.7%,97.7% and 97.2%,RSD were 0.9%,0.8%,1.0% and 1.1%,respectively.Conclusion： This method is accurate,precise and can be used for determination of all these substances in the Bazheng Mixture.