不同区域淡色库蚊对杀虫剂的抗性检验研究

An inspection of Culex pipiens pallens to Sixe Pesticides in different regions

目的:了解金华市城区、郊区、农村和山区四个不同区域淡色库蚊对6种常用杀虫剂的抗药性水平,为研究蚊虫抗性产生机制及科学合理使用杀虫剂提供依据。方法:采用药液浸渍法,测定4龄期幼虫半数致死浓度(LC50)。结果:金华市各区域淡色库蚊对氯菊酯抗性倍数最高,均大于10倍,属中抗以上水平;对仲丁威和胺菊酯的抗性倍数较低,均小于4倍,属低抗水平。四个区域中,农村淡色库蚊对各种杀虫剂的抗性最高,其中对氯菊酯、高效氯氰菊酯和溴氰菊酯的抗性倍数均大于10倍。金华市不同区域间自然种群的抗性比较差别无统计学意义(F=0.583,P=0.678);自然种群对不同杀虫剂间抗性发展水平表现不一致,经方差分析差别有统计学意义(F=9.136,P=0.000)。结论:金华市淡色库蚊对6种常用杀虫剂产生了不同程度的抗性,对氯菊酯最高,对胺菊酯、仲丁威普遍敏感,应加强对菊酯类杀虫剂的抗性监测及科学合理使用。

Objective：To investigate the level of the resistance of Culex pipiens pallens to six insecticides in City,suburbs,rural and mountainous,providing the basis for the study of mosquito resistance produced mechanism and scientific and reasonable use of pesticides.Methods：Immersion method of pesticide was used to assay the medium lethal concentration（LC50） of the Ⅳ larvae.Results：The Culex pipiens pallens were most resistant to permethrin which the resistance ratios were all above 10 times in each area,there were higher than medium resistance.The resistances to fenobucarb and tetramethrin were lower than other usual pesticides,the mosquitoes showed the lower resistance（RR〈4）.The resistance of rural was the highest to all sorts of insecticides in different regions.No statistical difference was found in the resistance between the natural populations from different regions（F=0.583,P=0.678）.Analysis of variance showed statistical difference in the varied insecticidal resistance of the natural populations to different agents（F=9.136,P=0.000）.Conclusion：The Culex pipiens pallens demonstrated varied resistance to the 6 pesticides,and were most resistant to permethrin.On the contrary,there were no insecticide resistance against fenobucarb and tetramethrin.The monitoring of its insecticide resistance should be strengthened in conjunction with proper application of insecticides in order to delay resistance evolution.