非小细胞肺癌组织ZO-1蛋白表达检测及其临床意义

Analysis and clinical significance of ZO-1 protein in patients with NSCLC

目的:探讨NSCLC(Non Small Cell Lung Cancer,非小细胞肺癌)患者中ZO-1(zonula occluden-1,紧密连接蛋白-1)蛋白检测的临床意义。方法:应用western blot检测101名NSCLC患者癌组织及癌旁组织ZO-1蛋白的表达,以61名肺部良性病变患者作为对照。结果:ZO-1蛋白在癌组织中表达低于癌旁组织,差异具有显著统计学意义(P<0.01);ZO-1蛋白在癌旁组织中表达低于对照组,差异具有显著统计学意义(P<0.05)。癌旁组织ZO-1蛋白在淋巴结未转移组中表达高于淋巴结转移组,差异具有显著统计学意义(t=-4.44,P<0.01),在2年生存组中表达高于2年死亡组,差异具有显著统计学意义(t=3.62,P<0.01);癌组织中ZO-1蛋白在2年生存组中表达高于2年死亡组,差异具有显著统计学意义(t=2.52,P<0.05)结论:癌旁组织中ZO-1蛋白和NSCLC患者淋巴结转移明显相关,癌旁组织和癌组织中ZO-1蛋白和NSCLC患者2年生存率明显相关。

Objective：The objective of this study was to investigate the zonula occluden-1（ZO-1） protein in patients with Non Small Cell Lung Cancer（NSCLC） and to indentify this roles in pathogenesis,development and classification of NSCLC.Methods： The ZO-1 protein of 101 patients with NSCLC and 61 patients with benign lung disease were detected by western blot.Results： There was significant statistic differences between carcinoma tissue group and control group（P0.01）,carcinoma tissue group and adjacent tissue group（P0.01）,adjacent tissue group and control group（P〈0.05） separately.ZO-1 protein in adjacent tissues were significant statistic different between lymph node invasion group and lymph nodes without invasion group（t=-4.44,P0.01）.ZO-1 protein in adjacent tissues were significant statistic different between 2-year suvival group and 2-year death group（t=3.62,P〈0.05）.ZO-1 protein in carcinoma tissues were significant statistic different between two-year suvival group and two-year death group（t=2.52,P〈0.05）.Conclusion： There is a significant relationship between ZO-1 protein expression in adjacent tissue and lymph node invasion.There is a significant relationship between ZO-1 protein expression in carcinoma or adjacent tissue and two-year survival rate in NSCLC patients.