摘要
目的:建立HPLC法测定如意金黄散中姜黄素含量的方法。方法:色谱柱为Shimadzu VP-ODS柱(250 mm×4.6 mm,5μm),流动相为甲醇-9.6%冰醋酸(50:50),流速1.0 mL/min,检测波长为420 nm,柱温为25℃。结果:姜黄素在波长为0.4944~2.4720μg(r=0.9999)内呈良好的线性关系,回收率分别为98.84%(RSD=1.76%)。结论:本方法简便、准确。
Objective:To establish a method for determination of curcumin in Ruyi Jinhuang Power by HPLC.Methods:The sample was separated on the Shimadzu C18 column(250 mm×4.6 mm,5 μm) with methanol-9.6% glacial acetic acid(50:50) as the mobile phase.The flow rate was 1.0 mL/min.The detection wavelength was 420 nm and the column temperature was 25 ℃.Results:Curcumin had the good linear relationship in the range of 0.4944-2.4720 μg(r=0.9999).The average recovery rate was 98.84%(RSD=1.76%).Conclusion:The method is simple and accurate.
出处
《现代医药卫生》
2011年第10期1461-1462,共2页
Journal of Modern Medicine & Health