合体滋养层细胞微粒制备方法的研究

Study of syncytiotrophoblast microparticles' preparation method

目的研究合体滋养层细胞微粒(syncytiotrophoblast microparticles STBM)的体外制备方法,并检测其纯度。方法收集两只足月正常健康剖宫产孕妇胎盘,将胎盘组织用机械方法打碎,运用差速离心法体外制备STBM;以胎盘碱性磷酸酶(placental alkaline-phosphatase PALP)作为膜标志酶,采用酶联免疫分析法检测STBM制剂的PALP的含量,确定STBM制剂的纯度。结果正常健康剖宫产孕妇胎盘中PLAP含量为56μg/ml,由其制备而来的STBM中PALP含量为900μg/ml,STBM较相应的胎盘组织纯化了16.07倍。结论取材于足月正常健康剖宫产孕妇胎盘组织,运用机械打碎方法及差速离心法,可以制备出高纯度的STBM,该方法简便易行,可作为一种高效、经济的体外制备STBM的方法。

Objective：To study the way of preparing syncytiotrophoblast microparticles（STBM） in vitro,and detect it＇s purity.Methods： STBM were prepared from two placentas which were collected sterile,immediately after elective caesarean section,from two healthy,full term women by breaking up the placentas and using differential centrifugation.The enrichment of PALP protein in STBM compared with placental homogenates was used to assess the purity of STBM by ELISA.Results： The content of PALP in placentas is 56 μg/ml,and the content of PALP in STBM is 900μg/ml.The alkaline phosphatase activity in STBM was enriched 16.07 fold relative to the initial placenta homogenate.Conclusion： STBM which were prepared from two placentas collected sterile,immediately after elective caesarean section,from two healthy,full term women by breaking up the placentas and using differential centrifugation is available.The way is simple,efficient and economical.