血清中TNF-α和IL-10与原因不明复发性流产的关系研究

Study of TNF-α and IL-10 in Serum and the relationship with unexplained recurrent spontaneous abortion

目的本实验通过检测不明原因复发性流产（URSA）患者和健康早孕者外周血清中TNF-α及IL-10的水平,探讨TNF-α及IL-10与URSA是否存在相关性,以及在URSA发生发展中的可能途径。方法前瞻性研究2008-2010年在杭州市第一人民医院门诊患者。年龄25-35周岁,孕龄〈12周,将有≥3次自然流产的URSA患者作为研究组;将曾有正常生育史,本次要求行人工流产终止妊娠的早孕健康妇女作为对照组,分别选择30例。用ELISA法检测血清TNF-α及IL-10的水平,比较是否存在统计学差异。结果 1.血清中TNF-α在研究组中的水平明显增高,有显著统计学意义（P〈0.05）;2.血清中IL-10在两组中的差异无统计学意义（P〉0.05）。结论 1.血清中TNF-α可作为判断URSA的参考指标;2.TNF-α可能参与URSA的发生发展。

Objective：In this study,by detecting the TNF-α and IL-10 in Serum of Unexplained recurrent spontaneous abortion（URSA） and early pregnancy,we can preliminarily study the role of TNF-α and IL-10 in the genesis and development of URSA.Meantime,we can preliminarily learn the available ways of the occurrence of URSA.Methods：Prospective study Outpatients of Hangzhou First People′s Hospital in 2008-2010.Put those women whose ages are between 25-35 year＇s old,and gestational age 12 weeks together as a study group.For those URSA patients who have been spontaneously aborted for ≥ 3 times,we regard this group as study group.While for those healthy patients who have normal reproductive history,demanding induced abortion in early pregnancy termination of pregnancy we will distinguished them as a control group.We select both URSA patients（study group） and healthy pregnant patients（control group） with 30 cases.We detect serum concentration TNF-α and IL-10 with ELISA,to compare the concentration differences of serum TNF-α and IL-10 of URSA and early pregnancy.Results： 1.Compared with the control group,concentration of TNF-α in the study group was significantly higher,the difference was statistically significant（P0.05）;2.The concentration of IL-10 was no significant difference（P0.05）.Conclusions：1.TNF-α could be used as a reference of URSA;2.Maybe TNF-α causes URSA.